Odom O W, Dabbs E R, Dionne C, Müller M, Hardesty B
Eur J Biochem. 1984 Jul 16;142(2):261-7. doi: 10.1111/j.1432-1033.1984.tb08280.x.
The apparent distances between probes covalently attached to the cysteine thiols of S1 or S21 and the 3' end of 16S RNA in Escherichia coli 30S ribosomal subunits were determined by non-radiative energy transfer to be: S21-16S RNA, 5.1 nm; S21-S1, 6.9 nm; S1-16S RNA, 6.8 nm. Binding of poly(uridylic acid) to 30S subunits causes the apparent distances between S1 and 16S RNA or S21 and 16S RNA to increase by more than 1.2 nm and 0.5 nm, respectively, but has little or no effect on the S1-S21 distance. Binding of 50S subunits causes an apparent increase in the S21-16S RNA and S21-S1 distances by 1.0 nm and 0.8 nm, respectively, but has little or no effect on the S1-16S-RNA distance.
通过非辐射能量转移测定了共价连接到大肠杆菌30S核糖体亚基S1或S21的半胱氨酸硫醇上的探针与16S RNA的3'端之间的表观距离，结果如下：S21-16S RNA为5.1纳米；S21-S1为6.9纳米；S1-16S RNA为6.8纳米。聚（尿苷酸）与30S亚基的结合分别使S1与16S RNA或S21与16S RNA之间的表观距离增加超过1.2纳米和0.5纳米，但对S1-S21距离几乎没有影响。50S亚基的结合分别使S21-16S RNA和S21-S1距离表观增加1.0纳米和0.8纳米，但对S1-16S-RNA距离几乎没有影响。
