大肠杆菌细胞中磷酸化蛋白质的二维分析。

Two-dimensional analysis of proteins phosphorylated in E. coli cells.

作者信息

Desmarquets G, Cortay J C, Cozzone A J

出版信息

FEBS Lett. 1984 Aug 6;173(2):337-41. doi: 10.1016/0014-5793(84)80801-7.

Abstract

Proteins phosphorylated in Escherichia coli cells were analyzed by the O'Farrell two-dimensional gel technique. Cytoplasmic and ribosomal fractions were studied separately. Double labeling with [32P]orthophosphate and [35S]sulfate followed by selective autoradiographic detection of each radioisotope allowed precise location of 12 major phosphoproteins on the total protein pattern of bacteria. Both the molecular mass and isoelectric point of these phosphoproteins were determined.

摘要

采用奥法雷尔双向凝胶技术分析了大肠杆菌细胞中磷酸化的蛋白质。分别研究了细胞质和核糖体部分。用[32P]正磷酸盐和[35S]硫酸盐进行双重标记，然后对每种放射性同位素进行选择性放射自显影检测，从而在细菌的总蛋白质图谱上精确确定了12种主要磷酸化蛋白质的位置。测定了这些磷酸化蛋白质的分子量和等电点。