奥法雷尔双向凝胶上的蛋白质鉴定：另外55种大肠杆菌蛋白质的定位
Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins.
作者信息
Phillips T A, Bloch P L, Neidhardt F C
出版信息
J Bacteriol. 1980 Dec;144(3):1024-33. doi: 10.1128/jb.144.3.1024-1033.1980.
Abstract
The resolution of proteins from whole-cell homogenates by two-dimensional gel electrophoresis is sufficiently reproducible and precise to permit different laboratories to exchange information about them. To the previous total of 81 we add the locations of 55 Escherichia coli proteins determined with the aid of purified proteins and mutant strains supplied by many investigators. The criteria used to establish the identifications of protein spots include migration with marker proteins, altered position or amount in appropriate mutant or plasmid-carrying strains, physiological behavior, and peptide map pattern.
摘要
通过二维凝胶电泳从全细胞匀浆中分离蛋白质，其结果具有足够的可重复性和精确性，使得不同实验室能够就这些蛋白质交换信息。在之前已确定的81种蛋白质的基础上，我们又补充了55种大肠杆菌蛋白质的位置信息，这些信息是借助许多研究人员提供的纯化蛋白质和突变菌株确定的。用于确定蛋白质斑点身份的标准包括与标记蛋白的迁移情况、在合适的突变体或携带质粒的菌株中位置或含量的改变、生理行为以及肽图谱模式。
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