Crocker P R, Blackwell J M, Bradley D J
Immunology. 1984 Jul;52(3):417-22.
Reciprocal radiation bone marrow chimaeras were made between H-2-compatible strains of mice innately resistant or susceptible to visceral leishmaniasis. In initial experiments, susceptibility but not resistance to Leishmania donovani could be transferred with donor bone marrow into irradiated recipients. In subsequent experiments it was possible to transfer both resistance and susceptibility. This was achieved either by selecting more radiosensitive mouse strains as susceptible recipients, or alternatively by increasing the irradiation dose for the susceptible recipients used in the initial experiments. Using the higher irradiation dose, successful transfer of resistance and susceptibility between congenic mice carrying the Lshr and Lshs alleles on the more radioresistant B10 genetic background provided firm evidence that the results obtained in this study were specifically related to expression of the Lsh gene. We conclude that Lsh gene-controlled resistance and susceptibility to L. donovani is determined by bone marrow-derived cells. The cell type(s) involved is likely to be of the macrophage lineage.
在对内脏利什曼病具有先天抗性或易感性的H-2兼容小鼠品系之间制作了相互辐射骨髓嵌合体。在最初的实验中，对杜氏利什曼原虫的易感性而非抗性可以通过供体骨髓转移到受辐照的受体中。在随后的实验中，抗性和易感性都可以转移。这可以通过选择对辐射更敏感的小鼠品系作为易感受体来实现，或者通过增加初始实验中使用的易感受体的辐照剂量来实现。使用更高的辐照剂量，在更具辐射抗性的B10遗传背景上携带Lshr和Lshs等位基因的同源小鼠之间成功转移抗性和易感性，为该研究中获得的结果与Lsh基因的表达具体相关提供了确凿证据。我们得出结论，Lsh基因控制的对杜氏利什曼原虫的抗性和易感性由骨髓来源的细胞决定。所涉及的细胞类型可能是巨噬细胞谱系。
