Eslami Z, Tanner C E
Institute of Parasitology of McGill University, Quebec, Canada.
Int J Parasitol. 1994 Aug;24(5):743-7. doi: 10.1016/0020-7519(94)90130-9.
A study was made of the proliferation dynamics in vitro of Leishmania donovani amastigotes in the resting peritoneal macrophages of C57BL/6 (Lshs) and C57L/J (Lshr) mice. Monolayers were inoculated with 5, 50 or 500 promastigotes per macrophage and the number of infected cells and the number of parasites per cell were determined 1, 3, 5, 7 and 14 days following inoculation. Results indicate that, irrespective of the phenotype of the donor mouse and of the inoculum, only 50-65% of the cells became infected initially. Expansion of the infection proceeded more rapidly in monolayers of Lshs cells and may have involved the "recruitment" of non-susceptible macrophages, perhaps by the action of a soluble factor. Also irrespective of the inoculum and phenotype, only 3-6 amastigotes were present in each macrophage initially, suggesting a limited number of ligands for the attachment of the parasite to the cell. Amastigotes did not proliferate for 3-4 days and then divided actively until day 7, when more parasites were present in the macrophages of the susceptible phenotype. Differential expansion of the infection and the proliferation of amastigotes in vitro suggest that resting peritoneal macrophages may, indeed, express the Lsh gene.
对杜氏利什曼原虫无鞭毛体在C57BL/6（Lshs）和C57L/J（Lshr）小鼠静息腹膜巨噬细胞中的体外增殖动力学进行了研究。每个巨噬细胞接种5、50或500个前鞭毛体形成单层，在接种后1、3、5、7和14天测定感染细胞数和每个细胞内的寄生虫数。结果表明，无论供体小鼠的表型和接种物如何，最初只有50 - 65%的细胞被感染。Lshs细胞单层中的感染扩展更快，可能涉及非易感巨噬细胞的“招募”，也许是通过一种可溶性因子的作用。同样，无论接种物和表型如何，每个巨噬细胞最初仅存在3 - 6个无鞭毛体，这表明寄生虫附着于细胞的配体数量有限。无鞭毛体在3 - 4天内不增殖，然后活跃分裂直至第7天，此时易感表型的巨噬细胞中有更多的寄生虫。感染的差异扩展和无鞭毛体在体外的增殖表明，静息腹膜巨噬细胞确实可能表达Lsh基因。
