Ceredig R, Schreyer M
Thymus. 1984;6(1-2):15-26.
The anatomical distribution of CBA (Thy-1.2) host and AKR (Thy-1.1) donor-derived cells in the regenerating thymus of AKR----CBA radiation bone marrow chimeras was investigated. Cryostat sections of chimeric thymuses were incubated with biotin-conjugated monoclonal anti-Thy-1 antibodies specific for host and donor-derived cells and the distribution of the corresponding Thy-1 antigen revealed by the immunoperoxidase staining technique. The thymus was initially repopulated by Thy-1.2+ host-derived cells, but by 28 days following bone marrow reconstitution the few remaining host cells were found mostly in the thymus medulla. However, occasional Thy-1.2+ cells were still present in extramedullary, primarily cortical, sites. Donor-derived (Thy-1.1+) cells were first seen in the 11-day chimeric thymus as single cells frequently closely associated with blood vessels in medullary areas. By 17 days, the cortex contained many Thy-1.1+ cells, although occasional single positive cells were still present in the medulla. Changes in the anatomical distribution of host and donor-derived cells in the regenerating chimeric thymus appeared to correlate with changes in their Thy-1 fluorescence profile as determined by flow microfluorometry.
研究了CBA（Thy-1.2）宿主和AKR（Thy-1.1）供体来源的细胞在AKR-CBA辐射骨髓嵌合体再生胸腺中的解剖分布。将嵌合胸腺的冷冻切片与针对宿主和供体来源细胞的生物素偶联抗Thy-1单克隆抗体孵育，并通过免疫过氧化物酶染色技术显示相应Thy-1抗原的分布。胸腺最初由Thy-1.2 +宿主来源的细胞重新填充，但在骨髓重建后28天，发现少数剩余的宿主细胞主要位于胸腺髓质中。然而，偶尔仍有Thy-1.2 +细胞存在于髓外，主要是皮质部位。供体来源的（Thy-1.1 +）细胞在11天的嵌合胸腺中首次出现，为单个细胞，常与髓质区域的血管紧密相关。到17天时，皮质中含有许多Thy-1.1 +细胞，尽管髓质中偶尔仍有单个阳性细胞。通过流式微荧光测定法确定，再生嵌合胸腺中宿主和供体来源细胞的解剖分布变化似乎与其Thy-1荧光谱的变化相关。
