通过流式细胞荧光测定法监测EMT6细胞指数期和平稳期的不同酯酶活性。
Different esterase activities of exponential and plateau phases of EMT6 cells monitored by flow cytofluorimetry.
作者信息
Watson J V, Workman P, Chambers S H
出版信息
Br J Cancer. 1978 Mar;37(3):397-402. doi: 10.1038/bjc.1978.59.
Abstract
The reaction rates of enzymes hydrolysing fluorescein diacetate have been studied in populations of intact tissue-culture EMT6, cells using flow cytofluorimetric techniques. It was found that the activity of these enzymes increased in plateau phases and that this correlated inversely with plating efficiency. Highly abnormal substrate-dependent reaction velocity kinetics were found in 14-, 21-, 28- and 35-day cultures.
摘要
使用流式细胞荧光测定技术，对完整的组织培养EMT6细胞群体中水解荧光素二乙酸酯的酶的反应速率进行了研究。发现这些酶的活性在平台期增加，并且这与接种效率呈负相关。在培养14天、21天、28天和35天的细胞中发现了高度异常的底物依赖性反应速度动力学。
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