Taylor I W, Bleehen N M
Br J Cancer. 1977 May;35(5):587-94. doi: 10.1038/bjc.1977.92.
The response of EMT6 mouse tumour cells to ICRF 159, both with and without X-radiation, has been measured during the life of monolayer cultures. The cytotoxic effect of ICRF 159 was found to be proliferation-dependent. Flow cytofluorimetry studies of cell cycle distribution showed that ICRF 159 prevented cell division while allowing DNA synthesis to continue. This anti-mitotic action and the cytotoxic effect of the drug were found to be closely related. Increased sensitivity to X-radiation was observed in cultures pretreated for 24 h with 200 microgram/ml ICRF 159 In exponential and early plateau cultures this was seen as a reduced shoulder of the survival curve. In late plateau cultures there was no apparent reduction of the shoulder, but an increase in slope.
在单层培养过程中，已对EMT6小鼠肿瘤细胞对ICRF 159（无论有无X射线照射）的反应进行了测量。发现ICRF 159的细胞毒性作用依赖于增殖。细胞周期分布的流式细胞荧光分析研究表明，ICRF 159可阻止细胞分裂，同时允许DNA合成继续进行。发现该药物的这种抗有丝分裂作用与细胞毒性作用密切相关。在用200微克/毫升ICRF 159预处理24小时的培养物中，观察到对X射线的敏感性增加。在指数期和早期平台期培养物中，这表现为存活曲线的肩部变窄。在晚期平台期培养物中，肩部没有明显变窄，但斜率增加。
