EMT6肿瘤细胞系单层培养过程中对辐射和ICRF 159敏感性的变化。
Changes in sensitivity to radiation and ICRF 159 during the life of monolayer cultures of EMT6 tumour line.
作者信息
Taylor I W, Bleehen N M
出版信息
Br J Cancer. 1977 May;35(5):587-94. doi: 10.1038/bjc.1977.92.
Abstract
The response of EMT6 mouse tumour cells to ICRF 159, both with and without X-radiation, has been measured during the life of monolayer cultures. The cytotoxic effect of ICRF 159 was found to be proliferation-dependent. Flow cytofluorimetry studies of cell cycle distribution showed that ICRF 159 prevented cell division while allowing DNA synthesis to continue. This anti-mitotic action and the cytotoxic effect of the drug were found to be closely related. Increased sensitivity to X-radiation was observed in cultures pretreated for 24 h with 200 microgram/ml ICRF 159 In exponential and early plateau cultures this was seen as a reduced shoulder of the survival curve. In late plateau cultures there was no apparent reduction of the shoulder, but an increase in slope.
摘要
在单层培养过程中，已对EMT6小鼠肿瘤细胞对ICRF 159（无论有无X射线照射）的反应进行了测量。发现ICRF 159的细胞毒性作用依赖于增殖。细胞周期分布的流式细胞荧光分析研究表明，ICRF 159可阻止细胞分裂，同时允许DNA合成继续进行。发现该药物的这种抗有丝分裂作用与细胞毒性作用密切相关。在用200微克/毫升ICRF 159预处理24小时的培养物中，观察到对X射线的敏感性增加。在指数期和早期平台期培养物中，这表现为存活曲线的肩部变窄。在晚期平台期培养物中，肩部没有明显变窄，但斜率增加。
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本文引用的文献
1
X-ray damage and recovery in mammalian cells in culture.培养的哺乳动物细胞中的X射线损伤与恢复
Nature. 1959 Oct 24;184:1293-5. doi: 10.1038/1841293a0.
2
Effect of ICRF159 on the mammalian cell cycle: significance for its use in cancer chemotherapy.ICRF159对哺乳动物细胞周期的影响：其在癌症化疗中的应用意义。
J Natl Cancer Inst. 1970 Mar;44(3):539-43.
3
Inhibition of metastatic spread by I.C.R.F. 159: selective deletion of a malignant characteristic.I.C.R.F. 159对转移扩散的抑制作用：恶性特征的选择性消除
Br Med J. 1970 Nov 7;4(5731):344-6. doi: 10.1136/bmj.4.5731.344.
4
Mode of action of the cytostatic agent "ICRF 159".细胞生长抑制剂“ICRF 159”的作用方式。
Nature. 1970 May 9;226(5245):524-6. doi: 10.1038/226524a0.
5
Cumulative cytostatic effect of ICRF 159.ICRF 159的累积细胞抑制作用。
Nature. 1974 Feb 15;247(5441):487-90. doi: 10.1038/247487a0.
6
7
8
Cell proliferation and the action of cytotoxic agents on haemopoietic tissue.细胞增殖以及细胞毒性药物对造血组织的作用。
Br J Haematol. 1972 Dec;23(6):751-8. doi: 10.1111/j.1365-2141.1972.tb03489.x.
9
Combined treatment of soft tissue and osteosarcomas by radiation and ICRF 159.软组织和骨肉瘤的放射治疗与ICRF 159联合治疗
Cancer. 1974 Oct;34(4):1040-5. doi: 10.1002/1097-0142(197410)34:4<1040::aid-cncr2820340413>3.0.co;2-5.
10
Synergism of ICRF 159 and radiotherapy in treatment of experimental tumors.ICRF 159与放射疗法在实验性肿瘤治疗中的协同作用。
Cancer. 1974 Oct;34(4):1033-9. doi: 10.1002/1097-0142(197410)34:4<1033::aid-cncr2820340412>3.0.co;2-0.
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