Tischler A S, Slayton V W, Costopoulos D S, Leape L L, DeLellis R A, Wolfe H J
Cancer. 1984 Oct 1;54(7):1344-7. doi: 10.1002/1097-0142(19841001)54:7<1344::aid-cncr2820540718>3.0.co;2-j.
Cells from two human neuroblastomas were found to exhibit a marked dependency on nerve growth factor (NGF) for survival in primary cultures. Cells from both tumors also responded to NGF by forming processes, but survival was not necessarily associated with process outgrowth. Lines of replicating cells could not be obtained from either tumor. NGF-dependent survival has not previously been reported as a characteristic of NGF-responsive human neuroblastoma cells in primary cultures or in cultures of established cell lines. Our findings suggest that tumors which require NGF for survival might constitute a biologically distinctive subset of neuroblastomas, or that NGF might function as a survival factor for some human neuroblastomas only in suboptimal or deleterious environments.
在原代培养中，发现来自两种人类神经母细胞瘤的细胞在存活方面对神经生长因子（NGF）表现出明显的依赖性。两种肿瘤的细胞对NGF也有反应，会形成突起，但存活并不一定与突起生长相关。从这两种肿瘤中均无法获得可复制的细胞系。此前尚未报道过在原代培养或已建立的细胞系培养中，NGF依赖性存活是NGF反应性人类神经母细胞瘤细胞的一个特征。我们的研究结果表明，需要NGF才能存活的肿瘤可能构成神经母细胞瘤中一个生物学上独特的亚群，或者NGF可能仅在次优或有害环境中才作为某些人类神经母细胞瘤的存活因子发挥作用。
