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莫洛尼氏鼠肉瘤病毒的转化蛋白是一种可溶性细胞质蛋白。

The transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein.

作者信息

Papkoff J, Nigg E A, Hunter T

出版信息

Cell. 1983 May;33(1):161-72. doi: 10.1016/0092-8674(83)90345-8.

DOI:10.1016/0092-8674(83)90345-8
PMID:6380747
Abstract

The transforming gene, v-mos, of Moloney murine sarcoma virus (M-MuSV) encodes a 37,000-dalton phosphoprotein, p37mos. Since the biochemical function of this protein is unknown, we have determined the subcellular location of p37mos in M-MuSV 124-transformed cells. Using two different methods of cell lysis and fractionation, we found that newly synthesized as well as mature p37mos is a soluble cytoplasmic protein. In agreement with these results, immunofluorescent staining of cells acutely infected with M-MuSV 124, using an antiserum directed against a synthetic v-mos peptide, produced a diffuse cytoplasmic pattern. Gel filtration experiments and glycerol gradient sedimentation analysis suggest that the bulk of p37mos exists as a monomer and is not involved in a specific association with other cellular proteins. These properties of p37mos are different from those of other characterized retroviral transforming proteins.

摘要

莫洛尼氏鼠肉瘤病毒（M-MuSV）的转化基因v-mos编码一种37000道尔顿的磷蛋白，即p37mos。由于该蛋白的生化功能尚不清楚，我们确定了p37mos在M-MuSV 124转化细胞中的亚细胞定位。使用两种不同的细胞裂解和分级分离方法，我们发现新合成的以及成熟的p37mos都是可溶性细胞质蛋白。与这些结果一致，用针对合成v-mos肽的抗血清对急性感染M-MuSV 124的细胞进行免疫荧光染色，产生了弥漫性细胞质模式。凝胶过滤实验和甘油梯度沉降分析表明，大部分p37mos以单体形式存在，不参与与其他细胞蛋白的特异性结合。p37mos的这些特性与其他已表征的逆转录病毒转化蛋白不同。

相似文献

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The transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein.莫洛尼氏鼠肉瘤病毒的转化蛋白是一种可溶性细胞质蛋白。
Cell. 1983 May;33(1):161-72. doi: 10.1016/0092-8674(83)90345-8.
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Analysis of v-mos encoded proteins in cells transformed by several related murine sarcoma viruses.
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Detection of a transforming gene product in cells transformed by Moloney murine sarcoma virus.在莫洛尼氏鼠肉瘤病毒转化的细胞中检测一种转化基因产物。
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Detection of an 85,000-dalton phosphoprotein in ts110 murine sarcoma virus-infected cells with antiserum against a v-mos peptide.用抗v-mos肽抗血清检测ts110鼠肉瘤病毒感染细胞中的一种85,000道尔顿的磷蛋白。
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Use of site-specific antipeptide antibodies to perturb the serine kinase catalytic activity of p37mos.使用位点特异性抗肽抗体干扰p37mos的丝氨酸激酶催化活性。
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Use of the mouse mammary tumor virus long terminal repeat to promote steroid-inducible expression of v-mos.利用小鼠乳腺肿瘤病毒长末端重复序列促进v-mos的类固醇诱导表达。
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P85: a gag-mos polyprotein encoded by ts110 Moloney murine sarcoma virus.P85：由ts110莫洛尼氏鼠肉瘤病毒编码的一种gag-mos多蛋白。
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Expression of the v-mos gene alters a Mr 55,000 protein during acute infection by Moloney murine sarcoma virus.在莫洛尼氏鼠肉瘤病毒急性感染期间，v-mos基因的表达会改变一种分子量为55,000的蛋白质。
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3629-33. doi: 10.1073/pnas.83.11.3629.

引用本文的文献

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Identification of an autoinhibitory region in the activation loop of the Mos protein kinase.在Mos蛋白激酶激活环中鉴定出一个自抑制区域。
Mol Cell Biol. 1996 Jul;16(7):3472-9. doi: 10.1128/MCB.16.7.3472.
2
Molecular cloning and characterization of a cDNA showing negative regulation in v-src-transformed 3Y1 rat fibroblasts.一个在v-src转化的3Y1大鼠成纤维细胞中呈负调控的cDNA的分子克隆与特性分析
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2593-7. doi: 10.1073/pnas.90.7.2593.
3
Ovarian teratomas in mice lacking the protooncogene c-mos.缺乏原癌基因c-mos的小鼠中的卵巢畸胎瘤。
Jpn J Cancer Res. 1995 Jun;86(6):540-5. doi: 10.1111/j.1349-7006.1995.tb02432.x.
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Nucleotide sequence of the feline retroviral oncogene v-fms shows unexpected homology with oncogenes encoding tyrosine-specific protein kinases.猫逆转录病毒致癌基因v-fms的核苷酸序列与编码酪氨酸特异性蛋白激酶的致癌基因显示出意外的同源性。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):85-9. doi: 10.1073/pnas.81.1.85.
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Comparison of myeloproliferative sarcoma virus with Moloney murine sarcoma virus variants by nucleotide sequencing and heteroduplex analysis.通过核苷酸测序和异源双链分析对骨髓增殖性肉瘤病毒与莫洛尼鼠肉瘤病毒变体进行比较。
J Virol. 1984 Jun;50(3):725-32. doi: 10.1128/JVI.50.3.725-732.1984.
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Complete c-mos (rat) nucleotide sequence: presence of conserved domains in c-mos proteins.完整的c-mos（大鼠）核苷酸序列：c-mos蛋白中保守结构域的存在
Nucleic Acids Res. 1984 Feb 24;12(4):2147-56. doi: 10.1093/nar/12.4.2147.
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Detection of c-abl tyrosine kinase activity in vitro permits direct comparison of normal and altered abl gene products.体外检测c-abl酪氨酸激酶活性可直接比较正常和变异的abl基因产物。
Mol Cell Biol. 1985 Nov;5(11):3116-23. doi: 10.1128/mcb.5.11.3116-3123.1985.

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