Muso E, Sekita K, Doi T, Kuwahara T, Yoshida H, Tamura T, Kawai C, Hamashima Y
Clin Immunol Immunopathol. 1984 Sep;32(3):351-8. doi: 10.1016/0090-1229(84)90278-2.
By a direct immunofluorescent technique, glomerular C3d deposition was examined in a total of 50 renal biopsy specimens from patients with lupus nephritis. C3d deposition was then compared with disease activity, glomerular IgG and C3c deposition, and the levels of circulating immune complexes (CIC) measured by a solid-phase anti-C3d assay. There was a good correlation between disease activity and the positivity of glomerular C3d deposits (P less than 0.001), as well as C3c deposits (P less than 0.001). Even in clinically inactive patients, a relatively high percentage (59%) of C3d deposits were positive compared with C3c deposits (17%). Mesangial C3d deposition correlated with clinical disease activity more significantly (P less than 0.005) than capillary wall C3d deposition (P less than 0.025). C3d deposits were detected in all of the 30 cases with positive C3c deposits, and moreover, in 15 of the 20 (75%) cases with negative C3c deposits. Glomerular IgG deposits were almost always associated with C3d deposits, both in mesangial areas and along capillary walls, with statistical significance (P less than 0.005, P less than 0.001, respectively). The serum levels of C3d-fixing immune complexes (IC) were significantly correlated with the positivity and intensity of mesangial C3d deposits. This study demonstrates glomerular deposition of C3d in patients with lupus nephritis and reveals a significant correlation between mesangial C3d deposition and disease activity.
采用直接免疫荧光技术，对50例狼疮性肾炎患者的肾活检标本进行肾小球C3d沉积检测。然后将C3d沉积与疾病活动度、肾小球IgG和C3c沉积以及通过固相抗C3d检测法测得的循环免疫复合物（CIC）水平进行比较。疾病活动度与肾小球C3d沉积阳性率（P<0.001）以及C3c沉积阳性率（P<0.001）之间存在良好的相关性。即使在临床无活动症状的患者中，C3d沉积阳性的比例（59%）也相对高于C3c沉积阳性的比例（17%）。系膜区C3d沉积与临床疾病活动度的相关性比毛细血管壁C3d沉积更显著（P<0.005比P<0.025）。在30例C3c沉积阳性的病例中均检测到C3d沉积，此外，在20例C3c沉积阴性的病例中有15例（75%）检测到C3d沉积。肾小球IgG沉积在系膜区和沿毛细血管壁几乎总是与C3d沉积相关，具有统计学意义（分别为P<0.005，P<0.001）。血清中C3d固定免疫复合物（IC）水平与系膜区C3d沉积的阳性率和强度显著相关。本研究证实了狼疮性肾炎患者肾小球中有C3d沉积，并揭示了系膜区C3d沉积与疾病活动度之间存在显著相关性。
