McCulloch E A
Clin Haematol. 1984 Jun;13(2):503-15.
Cell culture methods are available that permit colony formation by the normal progenitors of myelopoiesis and lymphopoiesis. The blast cells of acute myeloblastic leukaemia may also form colonies under selective conditions in culture. It is feasible, therefore, to determine some of the properties of blast progenitors. These were found to be uniformly in active cell cycle. However, great patient-to-patient variation was seen in the sensitivity of blast progenitors to chemotherapeutic agents and in their capacity to undergo self-renewal. The latter attribute, but not the former, was correlated with clinical outcome. These culture findings were used to propose a model of AML that includes an increase in diversity with malignant transformation. The model finds further support from studies of the phenotypes of blast cells freshly obtained from AML patients.
现有细胞培养方法可使髓系造血和淋巴细胞生成的正常祖细胞形成集落。急性髓性白血病的原始细胞在培养的选择性条件下也可形成集落。因此，确定原始祖细胞的一些特性是可行的。发现这些细胞均处于活跃的细胞周期。然而，原始祖细胞对化疗药物的敏感性及其自我更新能力在患者之间存在很大差异。后一种特性而非前一种特性与临床结果相关。这些培养结果被用于提出一种急性髓性白血病模型，该模型包括恶性转化过程中多样性的增加。该模型从对急性髓性白血病患者新鲜获取的原始细胞表型的研究中获得了进一步支持。
