Chang L J, Till J E, McCulloch E A
J Cell Physiol. 1980 Feb;102(2):217-22. doi: 10.1002/jcp.1041020213.
Blast cells from patients with Acute Myeloblastic Leukemia (AML) were separated according to cell size using velocity sedimentation under unit gravity. Fractions obtained in this way were plated in methyl cellulose with a growth stimulator present in media conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM). Colonies of blast cells form under these conditions. Pooled cell suspensions from such colonies were plated in microwells; the plating efficiency of such suspensions is a measure of blast progenitor self-renewal occurring in the original blast colonies. Self-renewal assays on each fraction indicated that self renewal among blast progenitors is heterogeneously distributed with subpopulations differing in renewal capacities. The results are consistent with the view that blast cell subpopulations in AML undergo a series of transitions associated with decreasing self renewal capacity, analogous to that observed in normal hemopoiesis, where proliferative capacity decreases with increasing differentiation.
利用单位重力下的速度沉降法，根据细胞大小对急性髓细胞白血病（AML）患者的原始细胞进行分离。以这种方式获得的组分接种于含有生长刺激因子的甲基纤维素中，该生长刺激因子存在于经植物血凝素（PHA-LCM）刺激的白细胞条件培养基中。在这些条件下，原始细胞形成集落。将来自此类集落的混合细胞悬液接种于微孔板中；此类悬液的接种效率是原始原始细胞集落中原始祖细胞自我更新能力的一种衡量指标。对每个组分进行的自我更新分析表明，原始祖细胞中的自我更新呈异质性分布，不同亚群的更新能力存在差异。这些结果与以下观点一致，即AML中的原始细胞亚群经历了一系列与自我更新能力下降相关的转变，这类似于在正常造血过程中观察到的情况，即增殖能力随着分化程度的增加而降低。
