Light N D, Tanner M J
Biochim Biophys Acta. 1978 Apr 20;508(3):571-6. doi: 10.1016/0005-2736(78)90101-3.
Reticulocytes of increasing maturity were separated by dextran gradient centrifugation. The accumulation in the membrane of the anion transport protein and other erythrocyte membrane proteins was studied during reticulocyte maturation by separating reticulocytes after incubation with [35S]-methionine. The incorporation of the reticulocyte membrane proteins was shown to be sequential, the anion transport protein being inserted at a very early stage in the cells' maturation.
通过葡聚糖梯度离心法分离出成熟度不断增加的网织红细胞。在用[35S]-甲硫氨酸孵育后，通过分离网织红细胞，研究了阴离子转运蛋白和其他红细胞膜蛋白在网织红细胞成熟过程中在膜中的积累情况。结果表明，网织红细胞膜蛋白的掺入是按顺序进行的，阴离子转运蛋白在细胞成熟的很早阶段就被插入。
