用干涉折射仪观察磷脂酰胆碱分散体系中的相变。
Phase transitions in phosphatidylcholine dispersion observed with an interference refractometer.
作者信息
Behof A F, Koza R A, Lach L E, Yi P N
出版信息
Biophys J. 1978 Apr;22(1):37-48. doi: 10.1016/S0006-3495(78)85469-1.
An interferometer is used to measure the refractive index change accompanying the crystal-to-liquid-crystal phase transition in the dispersion of phosphatidylcholines. Two separate methods of obtaining the refractive index change are employed: the first method analyzes the intensity transmitted through a spatial filter and the second method utilizes a piezoeletric crystal-based electronic compensator. The results of the two methods agree well. The accuracy of the apparatus (6 X 10(-6)) permitted us to use a very dilute sample to detect the phase change. Only a fraction of a milligram of dry lecithin is needed to observe the change. The result confirms conclusively that the major reason for the turbidity change at the transition temperature is the alteration in the refractive index of the lipid membranes. The fractional change in the refractive index does not agree well with the fractional change in the density of lipid molecules in vesicles.
干涉仪用于测量磷脂分散体系中伴随晶体向液晶相变的折射率变化。采用了两种独立的获取折射率变化的方法：第一种方法分析通过空间滤波器传输的强度，第二种方法利用基于压电晶体的电子补偿器。两种方法的结果吻合得很好。该仪器的精度（6×10⁻⁶）使我们能够使用非常稀的样品来检测相变。仅需几分之一毫克的干燥卵磷脂就能观察到这种变化。结果确凿地证实，转变温度下浊度变化的主要原因是脂质膜折射率的改变。折射率的分数变化与囊泡中脂质分子密度的分数变化不太一致。
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