Blomquist G, Ström G, Strömquist L H
Scand J Work Environ Health. 1984 Apr;10(2):109-13. doi: 10.5271/sjweh.2357.
The paper discusses a method developed for determining airborne fungi particles in environments highly contaminated with mold fungi. The collection of airborne fungi was performed with two slit samplers. These sampling devices were found to give the highest values out of the three different types tested simultaneously. After spores had been collected with the slit samplers, the collection medium, an agar gel, was removed from the petri dishes and homogenized in a sterile 0.9% sodium chloride solution. The homogenate was diluted stepwise and spread on agar plates prior to the cultivation and the determination of viable counts. When the homogenization procedure was tested on samples collected from three different work environments, no increase or decrease in the number of colony-forming units could be detected. Storage of the homogenate at 2 degrees C over 9 d did not increase the number of viable fungal colonies.
本文讨论了一种用于测定霉菌高度污染环境中空气传播真菌颗粒的方法。使用两个狭缝采样器收集空气传播真菌。在同时测试的三种不同类型的采样设备中，发现这些采样设备给出的值最高。用狭缝采样器收集孢子后，将收集介质（琼脂凝胶）从培养皿中取出，并在无菌0.9%氯化钠溶液中匀浆。匀浆逐步稀释，在培养和测定活菌数之前铺在琼脂平板上。当对从三个不同工作环境收集的样品进行匀浆程序测试时，未检测到菌落形成单位数量的增加或减少。将匀浆在2℃下储存9天并未增加活真菌菌落的数量。
