Sugiura M, Shiomi T, Nasu S
Yale J Biol Med. 1983 Sep-Dec;56(5-6):753-60.
We determined whether the western X mycoplasma (WXM) isolated from Colladonus montanus could be maintained in vitro by ultrathin sections or by assay of infectivity. Large spherical or small electron-dense bodies like those found in intact infected cells were observed in some media. Infectivity of WXM can be maintained for 28 days in cultured salivary glands in a newly developed medium, and for 281 days (seven passages) in modified AcTc, for 231 days (eight passages) in modified PC, 107 days (one passage) in spiroplasma medium, and 52 days (one passage) in modified GITC medium extracts. However, there is no evidence that WXM multiplied in any medium.
我们确定了从山地科拉多蝽分离出的西部X支原体（WXM）能否通过超薄切片或感染性测定在体外维持培养。在某些培养基中观察到了一些类似完整感染细胞中发现的大球形或小电子致密体。在一种新开发的培养基中，WXM在培养的唾液腺中的感染性可维持28天，在改良的AcTc中可维持281天（七代），在改良的PC中可维持231天（八代），在螺旋体培养基中可维持107天（一代），在改良的GITC培养基提取物中可维持52天（一代）。然而，没有证据表明WXM在任何培养基中增殖。
