Kozulić B, Barbarić S, Ries B, Mildner P
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1083-90. doi: 10.1016/0006-291x(84)91202-6.
It has been found that the carbohydrate part of acid phosphatase from yeast Saccharomyces cerevisiae consists of 16 N-glycosidically linked carbohydrate chains containing from 14 to about 150 mannose units. The presence of very small amounts of O-glycosidically linked chains was indicated. Acetolysis studies pointed to a high similarity in the structure of acid phosphatase and mannan carbohydrate chains. A new method is described for cross-linking of acid phosphatase specifically via carbohydrate chains. The possibility to cross-link the enzyme subunits intramolecularly is in accordance with the suggestion that carbohydrate chains play a role in subunit associations.
已发现来自酿酒酵母的酸性磷酸酶的碳水化合物部分由16条N-糖苷键连接的碳水化合物链组成，这些链含有14至约150个甘露糖单元。有迹象表明存在极少量的O-糖苷键连接的链。乙酰解研究表明酸性磷酸酶和甘露聚糖碳水化合物链的结构高度相似。描述了一种通过碳水化合物链特异性交联酸性磷酸酶的新方法。酶亚基分子内交联的可能性与碳水化合物链在亚基缔合中起作用的观点一致。
