Romanov G A, Romanova N A, Rozen V B, Vanyushin B F
Biochem Int. 1983 Mar;6(3):339-48.
The binding of 10(3)-fold purified rat liver glucocorticoid receptors to natural and synthetic polynucleotides has been studied. The receptors may bind to RNA as well as to DNA. The binding effectiveness of nucleic acids is strongly base composition dependent, with natural DNAs (double-stranded or denatured) greater than E. coli rRNA greater than poly(dA).poly(dT) greater than or equal to poly d(A,T,G)7 greater than or equal to poly(U) greater than poly(A).poly(U) greater than poly(A) approximately poly(C). The equilibrium (apparent) constants of receptor binding to a number of polynucleotides were calculated by a method proposed.
对经过10³倍纯化的大鼠肝脏糖皮质激素受体与天然和合成多核苷酸的结合进行了研究。这些受体既能与RNA结合，也能与DNA结合。核酸的结合效力强烈依赖于碱基组成，天然DNA（双链或变性）大于大肠杆菌rRNA大于聚（dA）·聚（dT）大于或等于聚（dA，T，G）7大于或等于聚（U）大于聚（A）·聚（U）大于聚（A）约等于聚（C）。通过所提出的一种方法计算了受体与多种多核苷酸结合的平衡（表观）常数。
