Luksha G L, Vaniushin B F
Biokhimiia. 1984 May;49(5):857-60.
Isolated nuclei from liver and brain of rats of various age bind isolated and 1000-2000-fold purified glucocorticoid-receptor complexes (GRC) containing [3H]dexamethasone. The binding of GRC by the nuclei decreases with an increase in the NaCl concentration from 0 to 0.4 M in a medium. The maximal degree of GRC binding is observed in the liver and brain nuclei of newborn rats (1-day-old); the nuclei of adult animals aged 3 months bind GRC 1/2 times as much. in old (25-month-old) and grown-up animals, no significant differences in GRC binding by nuclei from respective tissues were revealed. Brain nuclei of newborn rats were shown to bind GRC to a greater extent than liver nuclei.
从不同年龄大鼠的肝脏和大脑中分离出的细胞核，能结合含有[3H]地塞米松的分离的以及经过1000 - 2000倍纯化的糖皮质激素受体复合物（GRC）。在培养基中，随着氯化钠浓度从0增加到0.4M，细胞核对GRC的结合能力下降。新生大鼠（1日龄）的肝脏和大脑细胞核中观察到GRC结合的最大程度；3个月大的成年动物的细胞核结合GRC的量是新生大鼠的1/2。在老年（25个月大）和成年动物中，未发现相应组织的细胞核在GRC结合上有显著差异。新生大鼠的大脑细胞核比肝脏细胞核结合GRC的程度更高。
