Banwell J G, Abramowsky C R, Weber F, Howard R, Boldt D H
Dig Dis Sci. 1984 Oct;29(10):921-9. doi: 10.1007/BF01312481.
A purified plant lectin, phytohemagglutinin (PHA), or crude red kidney bean (RKB) from which it was derived, when incorporated as 1% of dietary protein into a purified casein protein diet caused weanling rats to fail to grow or lose weight in comparison to control animals pair fed an isonitrogenous, isocaloric diet. Feeding PHA was observed to cause diarrhea: fecal wet and dry weights were increased within 2 days after starting the diet. Increased fecal weight was caused by increased dry weight as well as by an increased fecal water content. On reversion to a normal casein diet, rapid amelioration of the antinutritional effects of PHA occurred with resumption of normal growth rate. Specific binding of PHA to the microvillus region of the small intestinal epithelium was demonstrated using rabbit anti-PHA and fluorescein-labeled goat anti-rabbit immunoglobin. PHA binding was observed after chronic intake in the diet or when applied to normal tissue in vitro. Loss of PHA binding to the intestine was observed to occur within 48 hr on reversion to a control casein diet. No significant morphological damage to the microvilli or the mucosal villus architecture was observed to accompany PHA adherence under these experimental conditions. Antinutritional and antiabsorptive effects of dietary PHA were associated with diarrhea. PHA adhered to the microvillus membrane of the small intestinal villus surface during the diarrheal state.(ABSTRACT TRUNCATED AT 250 WORDS)
一种纯化的植物凝集素——植物血凝素（PHA），或其来源的粗制红芸豆（RKB），当作为膳食蛋白质的1%掺入纯化的酪蛋白饮食中时，与喂食等氮、等热量对照饮食的同龄大鼠相比，导致断奶大鼠生长停滞或体重减轻。观察到喂食PHA会引起腹泻：开始饮食后2天内粪便湿重和干重增加。粪便重量增加是由干重增加以及粪便含水量增加所致。恢复正常酪蛋白饮食后，PHA的抗营养作用迅速改善，大鼠恢复正常生长速度。使用兔抗PHA和荧光素标记的山羊抗兔免疫球蛋白，证实了PHA与小肠上皮微绒毛区域的特异性结合。在长期饮食摄入或体外应用于正常组织后均观察到PHA结合。恢复对照酪蛋白饮食后48小时内，观察到PHA与肠道的结合消失。在这些实验条件下，未观察到PHA附着伴随微绒毛或黏膜绒毛结构出现明显形态损伤。膳食PHA的抗营养和抗吸收作用与腹泻有关。在腹泻状态下，PHA附着于小肠绒毛表面的微绒毛膜上。（摘要截短于250字）
