Steinemann C, Fenner M, Parish R W, Binz H
Int J Cancer. 1984 Sep 15;34(3):407-14. doi: 10.1002/ijc.2910340319.
Monoclonal antibodies against the mouse sarcoma FS9 were prepared. Antibodies recognizing a 37,000 dalton glycoprotein on FS9 tumor cells inhibit invasion by FS9 sarcoma cells of chicken heart fibroblasts in vitro as assessed by Abercrombie's confronted explant assay. Antibodies to other membrane proteins of FS9 tumor cells failed to inhibit invasiveness of FS9 sarcoma cells. The 37,000 dalton glycoprotein, which is neither a histocompatibility antigen nor a gp37 glycoprotein of Rous sarcoma virus nor the MEP described by Gottesman, is present on the surface and in the cytoplasm of FS9 sarcoma cells. The plasma membrane of the non-invasive mouse cell line L929 contains only low concentrations of the 37,000 dalton antigen. Hence, a relationship apparently exists between the increased concentration of this protein on the surface of FS9 cells and their invasiveness.
制备了针对小鼠肉瘤FS9的单克隆抗体。通过阿伯克龙比的对置外植体试验评估，识别FS9肿瘤细胞上一种37000道尔顿糖蛋白的抗体在体外可抑制FS9肉瘤细胞对鸡心脏成纤维细胞的侵袭。针对FS9肿瘤细胞其他膜蛋白的抗体未能抑制FS9肉瘤细胞的侵袭性。这种37000道尔顿的糖蛋白既不是组织相容性抗原，也不是劳氏肉瘤病毒的gp37糖蛋白，也不是戈特斯曼描述的MEP，它存在于FS9肉瘤细胞的表面和细胞质中。非侵袭性小鼠细胞系L929的质膜仅含有低浓度的37000道尔顿抗原。因此，FS9细胞表面这种蛋白质浓度的增加与其侵袭性之间显然存在某种关系。
