Steinemann C, Fenner M, Binz H, Parish R W
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3747-50. doi: 10.1073/pnas.81.12.3747.
Abercrombie 's confronted explant technique was used to study the role of tumor surface antigens in malignant invasion. Plasma membranes were isolated from mouse sarcoma cells ( FS9 ) and a mouse cell line (L929) of the same H-2 haplotype. FS9 cells are highly invasive when confronted with chicken heart fibroblasts, whereas the L929 cells are not [ Abercrombie , M. (1979) Nature (London) 281, 259-262]. The FS9 plasma membranes contained significantly higher concentrations of a 37,000-dalton glycoprotein. When antiserum directed against FS9 plasma membranes was preabsorbed with L929 cells, the antibodies remaining reacted predominantly with the 37,000-dalton antigen. Fab fragment prepared from the preabsorbed antiserum inhibited the invasion of chicken heart fibroblasts by FS9 cells. Fab prepared from a monoclonal antibody directed against the 37,000-dalton antigen also inhibited invasivity , whereas monoclonal antibodies reacting with two other FS9 cell surface antigens did not. The results imply a relationship between the increased concentration of the 37,000-dalton glycoprotein on the surface of the FS9 cells and their invasivity .
采用阿伯克龙比的体外植入技术研究肿瘤表面抗原在恶性侵袭中的作用。从具有相同H-2单倍型的小鼠肉瘤细胞（FS9）和小鼠细胞系（L929）中分离出质膜。当与鸡心脏成纤维细胞接触时，FS9细胞具有高度侵袭性，而L929细胞则不具有侵袭性[阿伯克龙比，M.（1979年）《自然》（伦敦）281，259 - 262]。FS9质膜中一种37000道尔顿的糖蛋白浓度显著更高。当用L929细胞预先吸收针对FS9质膜的抗血清时，剩余的抗体主要与37000道尔顿的抗原发生反应。从预先吸收的抗血清制备的Fab片段抑制了FS9细胞对鸡心脏成纤维细胞的侵袭。从针对37000道尔顿抗原的单克隆抗体制备的Fab也抑制了侵袭性，而与其他两种FS9细胞表面抗原发生反应的单克隆抗体则没有这种作用。结果表明FS9细胞表面37000道尔顿糖蛋白浓度的增加与其侵袭性之间存在关联。
