Hedman L A, Röckert L L, Lundin P M
Int J Immunopharmacol. 1984;6(4):357-63. doi: 10.1016/0192-0561(84)90055-9.
The corticosteroid effect on circulating lymphocytes in a steroid-sensitive species (rat) was studied by immunofluorescence. Both normal and neonatally thymectomized animals were injected with a high dose of prednisolone. T- and B-lymphocytes as well as T-lymphocyte subsets were analyzed in thoracic duct lymph during the involution phase (3 h after injection) and the restitution phase (17 h after injection) using conventional and monoclonal antisera. Neonatal thymectomy significantly reduced the number of circulating T-helper and T-non helper cells. In the involution phase, after a corticosteroid injection, there was a 64% reduction of lymph cell content in normal rats, affecting both T- and B-lymphocytes. Thymectomized animals showed an even more pronounced disappearance of cells. Counted in percentage, more B- than T-lymphocytes disappeared from the circulation. The T-non helper cells disappeared to a greater extent than the T-helper cells did. Cells with Ia-antigen showed about the same values as the B-lymphocytes. During the restitution phase all cells types were restored to normal levels. It seems that a high prednisolone dose has a more pronounced effect on the circulation of B- than of T-lymphocytes, and the T-non helper cells are probably more sensitive than the T-helper subpopulation. These data support the hypothesis that a major corticosteroid effect is a trapping and redistribution of circulating lymphocytes, more pronounced for certain cell types.
采用免疫荧光法研究了皮质类固醇对类固醇敏感物种（大鼠）循环淋巴细胞的影响。给正常和新生期胸腺切除的动物注射高剂量的泼尼松龙。在退化期（注射后3小时）和恢复阶段（注射后17小时），使用传统抗血清和单克隆抗血清分析胸导管淋巴液中的T淋巴细胞、B淋巴细胞以及T淋巴细胞亚群。新生期胸腺切除显著减少了循环中辅助性T细胞和非辅助性T细胞的数量。在退化期，注射皮质类固醇后，正常大鼠的淋巴细胞含量减少了64%，T淋巴细胞和B淋巴细胞均受影响。胸腺切除的动物细胞消失更为明显。按百分比计算，循环中消失的B淋巴细胞比T淋巴细胞更多。非辅助性T细胞比辅助性T细胞消失的程度更大。带有Ia抗原的细胞与B淋巴细胞表现出大致相同的值。在恢复阶段，所有细胞类型都恢复到正常水平。似乎高剂量的泼尼松龙对B淋巴细胞循环的影响比对T淋巴细胞更明显，并且非辅助性T细胞可能比辅助性T细胞亚群更敏感。这些数据支持了以下假说：皮质类固醇的主要作用是使循环淋巴细胞滞留和重新分布，对某些细胞类型的影响更为明显。
