Mortensen S B, Kilian M
J Chromatogr. 1984 Jul 27;296:257-62. doi: 10.1016/s0021-9673(01)96419-2.
A rapid assay to detect and quantitate immunoglobulin A1 (IgA1) protease activity was developed by the use of a high-performance gel-permeation chromatography column. The assay measured the disappearance of intact substrate and the emergence of cleavage fragments and the results could be expressed in absolute units. The utility of the assay was demonstrated in the partial purification of an IgA1 protease from a strain of Haemophilus influenzae.
通过使用高效凝胶渗透色谱柱，开发了一种快速检测和定量免疫球蛋白A1（IgA1）蛋白酶活性的方法。该方法测量完整底物的消失和裂解片段的出现，结果可以用绝对单位表示。该方法的实用性在从一株流感嗜血杆菌中部分纯化IgA1蛋白酶的过程中得到了证明。
