Nielsen K, Rosenbaum B, Ballinger R, Stiller J
Vet Immunol Immunopathol. 1984 Jul;6(3-4):273-83. doi: 10.1016/0165-2427(84)90053-9.
Bovine complement was treated with various agents known to activate or inactivate one or more of the cascade components. The treated complement was then assessed for remaining hemolytic activity by a tube titration test and a radial hemolysis method. Divalent cation chelators (EDTA and EGTA); immune complexes prepared with serum and IgM, IgG1, IgG2, and IgA isotypes; smooth and rough lipopolysaccharides and lipid A; hydrazine; zymosan; cobra venom factor and brown recluse spider venom caused depletion of complement as determined in the tube titration test. Immune complexes (prepared with serum); hydrazine; cobra venom factor; EDTA and smooth lipopolysaccharide caused loss of hemolytic activity in the radial hemolysis test. This evidence suggests that the radial hemolysis test assessed complement consumed by the alternate pathway, while the tube titration method measured classical pathway consumption.
用已知能激活或灭活一种或多种级联成分的各种试剂处理牛补体。然后通过试管滴定试验和放射状溶血法评估处理后的补体的剩余溶血活性。二价阳离子螯合剂（乙二胺四乙酸和乙二醇双四乙酸）；用血清以及IgM、IgG1、IgG2和IgA同种型制备的免疫复合物；光滑和粗糙的脂多糖以及脂质A；肼；酵母聚糖；眼镜蛇毒因子和棕色遁蛛毒液导致补体消耗，这在试管滴定试验中得到确定。免疫复合物（用血清制备）；肼；眼镜蛇毒因子；乙二胺四乙酸和光滑脂多糖导致放射状溶血试验中的溶血活性丧失。该证据表明，放射状溶血试验评估的是由替代途径消耗的补体，而试管滴定法测量的是经典途径的消耗。
