Fauci A S, Whalen G, Burch C
J Immunol. 1980 May;124(5):2410-3.
A system for the development of indirect (IgG, IgA, and IgM) anti-SRBC plaque-forming cells (PFC) after polyclonal stimulation of human peripheral blood lymphocytes with pokeweed mitogen (PWM) is described. Rabbit anti-human polyvalent Ig or Ig class-specific antisera were employed. Most of the indirect PFC were IgG with less being IgM and IgA. The finding of significantly more indirect IgM PFC than direct PFC suggests that many IgM PFC are producing low efficiency hemolysin that requires an anti-globulin to produce lysis. The system is T cell dependent and cultures require selected batches of supplemented fetal calf serum that preferentially yield indirect (1527 +/- 243 PFC/10(6) lymphocytes) as opposed to direct (36 +/- 11 PFC/10(6) lymphocytes) PFC. Batches of human AB sera that were previously chosen to yield optimal direct PFC (approximately 200 to 300 PFC/10(6) lymphocytes) did not yield indirect PFC. Hence, depending on culture conditions, polyclonal stimulation of human lymphocytes can yield antigen-specific direct and indirect hemolytic PFC.
本文描述了一种在用美洲商陆有丝分裂原（PWM）对人外周血淋巴细胞进行多克隆刺激后，用于培养间接（IgG、IgA和IgM）抗绵羊红细胞空斑形成细胞（PFC）的系统。使用了兔抗人多价Ig或Ig类特异性抗血清。大多数间接PFC为IgG，IgM和IgA较少。间接IgM PFC明显多于直接PFC的发现表明，许多IgM PFC产生的溶血素效率较低，需要抗球蛋白才能产生裂解。该系统依赖T细胞，培养需要选择特定批次的补充胎牛血清，这种血清优先产生间接（1527±243 PFC/10⁶淋巴细胞）而非直接（36±11 PFC/10⁶淋巴细胞）PFC。之前选择用于产生最佳直接PFC（约200至300 PFC/10⁶淋巴细胞）的人AB血清批次，不能产生间接PFC。因此，根据培养条件，人淋巴细胞的多克隆刺激可产生抗原特异性直接和间接溶血PFC。
