Chang J P, MacKenzie D S, Gould D R, Peter R E
Life Sci. 1984 Nov 12;35(20):2027-33. doi: 10.1016/0024-3205(84)90559-9.
Enzymatically dispersed goldfish pituitary cells or freshly prepared goldfish pituitary fragments continue to secrete gonadotropin spontaneously in a column perifusion system. After the establishment of basal secretion rates, treatment of dispersed pituitary cells with 5 and 500 nM dopamine, or pituitary fragments with 50 and 100 nM dopamine, decreased the amount of gonadotropin released into the perifusate. Perifusion with 500 nM dopamine also abolished the gonadotropin-release response to a 10 nM solution of a luteinizing hormone-releasing hormone analogue in both perifusion systems. Perifusion of pituitary dispersed cells or fragment preparations obtained from sexually regressed goldfish with 50 nM norepinephrine consistently increased the amount of gonadotropin released into the perifusate. These results provide in vitro evidence for direct dopamine inhibition of spontaneous gonadotropin release, blockade by dopamine of gonadotropin-releasing hormone actions, and norepinephrine stimulation of gonadotropin secretion in goldfish.
在柱式灌流系统中，经酶分散的金鱼垂体细胞或新鲜制备的金鱼垂体碎片会持续自发分泌促性腺激素。在建立基础分泌率后，用5和500 nM多巴胺处理分散的垂体细胞，或用50和100 nM多巴胺处理垂体碎片，会减少释放到灌流液中的促性腺激素量。在两种灌流系统中，用500 nM多巴胺灌流也会消除对10 nM促黄体生成素释放激素类似物溶液的促性腺激素释放反应。用50 nM去甲肾上腺素灌流从性消退金鱼获得的垂体分散细胞或碎片制剂，会持续增加释放到灌流液中的促性腺激素量。这些结果为多巴胺直接抑制金鱼促性腺激素的自发释放、多巴胺对促性腺激素释放激素作用的阻断以及去甲肾上腺素对金鱼促性腺激素分泌的刺激提供了体外证据。
