Tucker J D, Xu J, Stewart J, Ong T
Mutat Res. 1984 Oct;138(1):93-8. doi: 10.1016/0165-1218(84)90090-9.
A method using sister-chromatid exchanges (SCEs) for genotoxic testing of gaseous compounds is described. Human peripheral lymphocyte cultures previously stimulated with phytohemagglutinin were placed in sterile dialysis tubing and then put in an enclosed flask containing additional culture media. Air, with or without ethylene dibromide (EDB), was bubbled through the flask for up to 8 h. The cultures were harvested 75 h after culture initiation, and second-division cells were scored for induction of SCEs according to established procedures. The SCE frequency was approximately doubled in cultures treated with EDB. A similar experiment with air alone resulted in only slight increases in SCEs. The results indicate that this system is potentially useful for detecting genotoxicity of gases and vapors and may be useful for the detection of genotoxic agents in occupational settings.
描述了一种使用姐妹染色单体交换（SCEs）对气态化合物进行遗传毒性测试的方法。先前用植物血凝素刺激过的人外周血淋巴细胞培养物被置于无菌透析管中，然后放入一个装有额外培养基的封闭烧瓶中。含有或不含有二溴乙烷（EDB）的空气通过烧瓶鼓泡长达8小时。培养开始75小时后收获培养物，并根据既定程序对第二分裂期细胞进行SCEs诱导评分。用EDB处理的培养物中SCE频率大约增加了一倍。仅用空气进行的类似实验仅导致SCEs略有增加。结果表明，该系统可能有助于检测气体和蒸气的遗传毒性，并且可能有助于在职业环境中检测遗传毒性剂。
