对暴露于二溴乙烷的小鼠进行的体内细胞遗传学研究。

In vivo cytogenetic studies on mice exposed to ethylene dibromide.

作者信息

Krishna G, Xu J, Nath J, Petersen M, Ong T

出版信息

Mutat Res. 1985 Oct-Nov;158(1-2):81-7. doi: 10.1016/0165-1218(85)90101-6.

Abstract

The pesticide, ethylene dibromide (EDB), was evaluated with in vivo cytogenetic assays to determine its genotoxicity. CD1 male mice were exposed to EDB through intraperitoneal injections. Bone marrow cells isolated from femora were analyzed for sister-chromatid exchange (SCE), chromosome aberration and micronucleus formation. The results showed that only certain concentrations of EDB tested caused a slight but significant increase in SCEs and chromosome aberrations. However, these increases were not dose-related. No increase in the polychromatic erythrocytes with micronuclei was observed following EDB exposure. Also, EDB did not cause cell-cycle delay in comparison with controls. Thus, it appears that EDB is not an effective genotoxic agent in vivo in mice.

摘要

对农药二溴乙烷（EDB）进行了体内细胞遗传学试验，以确定其遗传毒性。通过腹腔注射将CD1雄性小鼠暴露于EDB。分析从股骨分离的骨髓细胞的姐妹染色单体交换（SCE）、染色体畸变和微核形成情况。结果表明，仅在所测试的某些浓度的EDB下，SCE和染色体畸变有轻微但显著的增加。然而，这些增加与剂量无关。暴露于EDB后，未观察到含微核的多染红细胞增加。此外，与对照组相比，EDB未导致细胞周期延迟。因此，似乎EDB在小鼠体内不是一种有效的遗传毒性剂。