Kon N D, Hansen K J, Martin M B, Meredith J W, Meredith J H, Cordell A R
Surgery. 1984 Nov;96(5):870-3.
The effects of ibuprofen (Motrin), dipyridamole (Persantine), and prostacyclin on the deposition of platelets on polytetrafluoroethylene grafts (5.5 cm long, 4 mm internal diameter implanted into both femoral arteries of 21 dogs) were studied by harvesting autologous platelets, labeling them with 51Cr, and reinjecting them 15 minutes before cross-clamping. Arterial flow was adjusted to 65 ml/min and monitored continuously. Ibuprofen (12.5 mg/kg) and dipyridamole (2.5 mg/kg) were each administered as a single intravenous injection in four dogs (eight grafts) and five dogs (10 grafts), respectively. Prostacyclin (50 ng/kg/min) was administered by continuous intravenous infusion in five dogs (10 grafts). Seven dogs (14 grafts) served as controls. Grafts were removed 2 hours after implantation, and radioactivity (counts per 10 minutes) was determined in four segments of each graft (including both anastomoses) with a gamma-counter. Counts were averaged for each group of dogs. Significance was calculated by the Student t test. Platelet deposition in control dogs averaged 10,033.9 +/- 1134.2 SE; that in prostacyclin-treated dogs averaged 2513.7 +/- 276 SE (p less than 0.001); that in ibuprofen-treated dogs averaged 5453.4 +/- 1336.3 SE (p = 0.02); that in dipyridamole-treated dogs averaged 11,213.7 +/- 1632.5 SE (p = 0.55). These data demonstrate the effectiveness of prostacyclin and ibuprofen in reducing platelet deposition on polytetrafluorethylene grafts in dogs.
通过采集自体血小板，用51Cr标记，在交叉钳夹前15分钟重新注入，研究了布洛芬（莫特林）、双嘧达莫（潘生丁）和前列环素对血小板在聚四氟乙烯移植物（长5.5厘米，内径4毫米，植入21只狗的双侧股动脉）上沉积的影响。将动脉血流调整至65毫升/分钟并持续监测。布洛芬（12.5毫克/千克）和双嘧达莫（2.5毫克/千克）分别对4只狗（8个移植物）和5只狗（10个移植物）进行单次静脉注射。前列环素（50纳克/千克/分钟）通过持续静脉输注给予5只狗（10个移植物）。7只狗（14个移植物）作为对照。植入后2小时取出移植物，用γ计数器测定每个移植物的四个部分（包括两个吻合口）的放射性（每10分钟计数）。计算每组狗的计数平均值。采用Student t检验计算显著性。对照狗的血小板沉积平均为10,033.9±1134.2标准误；前列环素治疗组狗的平均为2513.7±276标准误（p＜0.001）；布洛芬治疗组狗的平均为5453.4±1336.3标准误（p = 0.02）；双嘧达莫治疗组狗的平均为11,213.7±1632.5标准误（p = 0.55）。这些数据证明了前列环素和布洛芬在减少狗的聚四氟乙烯移植物上血小板沉积方面的有效性。
