Kutter E, Drivdahl R, Rand K
Genetics. 1984 Oct;108(2):291-304. doi: 10.1093/genetics/108.2.291.
Bacteriophage T4 infection rapidly and almost completely inhibits transcription of host and other phage DNAs. Two processes have been implicated to date in this inhibition: (1) ADP ribosylation of the alpha subunits of the RNA polymerase, involving gpalt (which is injected with the phage DNA) and, later, gpmod; and (2) the action of the T4 alc/unf gene product, synthesized immediately after infection. The latter unfolds the host genome and also blocks transcription of cytosine-containing DNA. Here, we describe the identification on two-dimensional polyacrylamide gels of gpalc/unf, the more precise mapping of the gene and the identification and analysis of the appropriate DNA sequence from an Unf+ alc mutant.
噬菌体T4感染能迅速且几乎完全抑制宿主及其他噬菌体DNA的转录。迄今为止，这种抑制作用涉及两个过程：（1）RNA聚合酶α亚基的ADP核糖基化，涉及gpalt（与噬菌体DNA一起注入）以及随后的gpmod；（2）感染后立即合成的T4 alc/unf基因产物的作用。后者使宿主基因组解折叠并阻断含胞嘧啶DNA的转录。在此，我们描述了在二维聚丙烯酰胺凝胶上对gpalc/unf的鉴定、该基因更精确的定位以及来自Unf + alc突变体的相应DNA序列的鉴定和分析。
