单克隆抗体检测大肠杆菌临床分离株中IncF组质粒编码的TraT蛋白
Monoclonal antibody detection of IncF group plasmid-encoded TraT protein in clinical isolates of Escherichia coli.
作者信息
Bitter-Suermann D, Peters H, Jürs M, Nehrbass R, Montenegro M, Timmis K N
出版信息
Infect Immun. 1984 Nov;46(2):308-13. doi: 10.1128/iai.46.2.308-313.1984.
The TraT protein specified by IncF group plasmids mediates surface exclusion and bacterial resistance to the lethal activities of serum. In this study, an anti-TraT protein monoclonal antibody was generated which failed to react with TraT+ bacteria but which efficiently detected solubilized TraT protein in Western blots and in an enzyme-linked immunosorbent assay. Use of this antibody to screen clinical and nonclinical isolates of Escherichia coli for the production of TraT protein revealed its presence in a modest proportion (38%) of normal fecal strains, a significantly higher proportion of clinical strains (51 to 73%), and an even higher proportion (78 to 88%) of clinical strains concomitantly producing the K1 capsule, an important virulence factor of E. coli.
IncF 族质粒所编码的 TraT 蛋白介导表面排斥以及细菌对血清致死活性的抗性。在本研究中，制备了一种抗 TraT 蛋白单克隆抗体，该抗体不能与 TraT⁺细菌发生反应，但在蛋白质免疫印迹法和酶联免疫吸附测定中能有效检测到可溶的 TraT 蛋白。使用该抗体筛选大肠杆菌的临床和非临床分离株以检测 TraT 蛋白的产生情况，结果显示其在一定比例（38%）的正常粪便菌株中存在，在临床菌株中的比例显著更高（51%至 73%），而在同时产生 K1 荚膜（大肠杆菌的一种重要毒力因子）的临床菌株中比例甚至更高（78%至 88%）。
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