Page M G, West I C
Biochem J. 1984 Nov 1;223(3):723-31. doi: 10.1042/bj2230723.
The uptake of galactosides into Escherichia coli via the lactose permease was studied in the time range 0.01-10s by rapid mixing and quenched flow. An initial transient was observed under two conditions. Firstly, a lag in the approach to the steady state was observed at low galactoside concentrations (less than Km). Secondly, a burst of uptake was observed when anaerobic cell suspensions were mixed with aerobic substrate solutions. However, the cause of the burst of uptake appears to be a burst in the rate of respiration. The rate of galactoside uptake during this phase is 10-fold greater than during the steady state.
通过快速混合和淬灭流动,在0.01 - 10秒的时间范围内研究了半乳糖苷通过乳糖通透酶进入大肠杆菌的过程。在两种条件下观察到了初始瞬态。首先,在低半乳糖苷浓度(低于Km)下,观察到达到稳态的过程存在滞后。其次,当厌氧细胞悬液与需氧底物溶液混合时,观察到摄取的爆发。然而,摄取爆发的原因似乎是呼吸速率的爆发。在此阶段半乳糖苷的摄取速率比稳态时高10倍。