大肠杆菌β-半乳糖苷/质子同向转运的替代底物抑制作用及动力学机制
Alternative-substrate inhibition and the kinetic mechanism of the beta-galactoside/proton symport of Escherichia coli.
作者信息
Page M G, Jou Y H
出版信息
Biochem J. 1982 Jun 15;204(3):681-8. doi: 10.1042/bj2040681.
Abstract
The effects of competing alternative substrates on the rate of uptake by galactoside/proton symport were investigated. These experiments produced a decrease in apparent maximum velocity with increased alternative-substrate concentration that cannot be accounted for by a simple ordered mechanism. This, together with non-linearities in the variation of the apparent kinetic constants with alternative-substrate concentration, can be accounted for by a random mechanism for galactoside and proton binding.
摘要
研究了竞争性替代底物对半乳糖苷/质子同向转运摄取速率的影响。这些实验表明，随着替代底物浓度的增加，表观最大速度降低，而简单的有序机制无法解释这种现象。表观动力学常数随替代底物浓度变化的非线性，以及上述现象，都可以用半乳糖苷和质子结合的随机机制来解释。
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2
[Kinetic studies on galactoside permease of Escherichia coli].[大肠杆菌半乳糖苷通透酶的动力学研究]
Biochim Biophys Acta. 1960 May 6;40:70-84. doi: 10.1016/0006-3002(60)91316-0.
3
THE ROLE OF PERMEASE IN TRANSPORT.通透酶在运输中的作用。
Biochim Biophys Acta. 1964 Jan 27;79:177-200. doi: 10.1016/0926-6577(64)90050-6.
4
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Biochem J. 1961 Aug;80(2):324-32. doi: 10.1042/bj0800324.
5
[Galactoside-permease of Escherichia coli].[大肠杆菌的半乳糖苷通透酶]
Ann Inst Pasteur (Paris). 1956 Dec;91(6):829-57.
6
Slip and leak models of gradient-coupled solute transport.梯度耦合溶质转运的滑移和渗漏模型
Biochem Soc Trans. 1980 Jun;8(3):271-3. doi: 10.1042/bst0080271.
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8
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