允许麦芽糖通过β-半乳糖苷转运系统进行转运的大肠杆菌K-12突变体。
Escherichia coli K-12 mutants that allow transport of maltose via the beta-galactoside transport system.
作者信息
Shuman H A, Beckwith J
出版信息
J Bacteriol. 1979 Jan;137(1):365-73. doi: 10.1128/jb.137.1.365-373.1979.
Abstract
We have isolated mutants of Escherichia coli that have an altered beta-galactoside transport system. This altered transport system is able to transport a sugar, maltose, that the wild-type beta-galactoside transport system is unable to transport. The mutation that alters the specificity of the transport system is in the lacY gene, and we refer to the allele as lacYmal. The lacYmal allele was detected originally in strains in which the lac genes were fused to the malF gene. Thus, as a result of gene fusion and isolation of the lacYmal mutation, a new transport system was evolved with regulatory properties and specificity similar to those of the original maltose transport system. Maltose transport via the lacYmal gene product is independent of all of the normal maltose transport system components. The altered transport system shows a higher affinity than the wild-type transport system for two normal substrates of the beta-galactoside transport system, thiomethyl-beta-D-galactoside and o-nitrophenyl-beta-D-galactoside.
摘要
我们已经分离出了大肠杆菌的突变体，其β-半乳糖苷转运系统发生了改变。这种改变后的转运系统能够转运一种糖——麦芽糖，而野生型β-半乳糖苷转运系统无法转运麦芽糖。改变转运系统特异性的突变位于lacY基因中，我们将该等位基因称为lacYmal。lacYmal等位基因最初是在lac基因与malF基因融合的菌株中检测到的。因此，由于基因融合和lacYmal突变的分离，进化出了一种新的转运系统，其调控特性和特异性与原始麦芽糖转运系统相似。通过lacYmal基因产物进行的麦芽糖转运不依赖于所有正常的麦芽糖转运系统成分。与野生型转运系统相比，改变后的转运系统对β-半乳糖苷转运系统的两种正常底物硫代甲基-β-D-半乳糖苷和邻硝基苯基-β-D-半乳糖苷具有更高的亲和力。
相似文献
1
Escherichia coli K-12 mutants that allow transport of maltose via the beta-galactoside transport system.允许麦芽糖通过β-半乳糖苷转运系统进行转运的大肠杆菌K-12突变体。
J Bacteriol. 1979 Jan;137(1):365-73. doi: 10.1128/jb.137.1.365-373.1979.
2
The lactose/H+ carrier of Escherichia coli: lac YUN mutation decreases the rate of active transport and mimics an energy-uncoupled phenotype.大肠杆菌的乳糖/H⁺载体：lac YUN突变降低了主动运输速率并模拟了能量解偶联表型。
Biochem J. 1985 Apr 1;227(1):287-97. doi: 10.1042/bj2270287.
3
Studies on beta-galactoside transport in a Proteus mirabilis merodiploid carrying an Escherichia coli lactose operon.对携带大肠杆菌乳糖操纵子的奇异变形杆菌部分二倍体中β-半乳糖苷转运的研究。
J Bacteriol. 1973 Oct;116(1):131-40. doi: 10.1128/jb.116.1.131-140.1973.
4
Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.钠离子和锂离子在大肠杆菌蜜二糖转运系统介导的硫代甲基半乳糖苷转运中的作用
J Bacteriol. 1978 Apr;134(1):147-56. doi: 10.1128/jb.134.1.147-156.1978.
5
Characterization of lactose carrier mutants which transport maltose.转运麦芽糖的乳糖载体突变体的特性分析
J Biol Chem. 1985 Dec 25;260(30):16181-6.
6
7
8
The kinetic mechanism of galactoside/H+ cotransport in Escherichia coli.大肠杆菌中半乳糖苷/H⁺共转运的动力学机制。
Biochim Biophys Acta. 1986 Mar 13;855(3):391-416. doi: 10.1016/0005-2736(86)90085-4.
9
Alternative-substrate inhibition and the kinetic mechanism of the beta-galactoside/proton symport of Escherichia coli.大肠杆菌β-半乳糖苷/质子同向转运的替代底物抑制作用及动力学机制
Biochem J. 1982 Jun 15;204(3):681-8. doi: 10.1042/bj2040681.
10
Interaction of maltose transport with the transport of glucose and galactosides.麦芽糖转运与葡萄糖和半乳糖苷转运的相互作用。
J Bacteriol. 1972 Jan;109(1):455-8. doi: 10.1128/jb.109.1.455-458.1972.
引用本文的文献
1
High-throughput synthetic rescue for exhaustive characterization of suppressor mutations in human genes.高通量合成拯救技术，用于全面表征人类基因中的抑制性突变。
Cell Mol Life Sci. 2020 Nov;77(21):4209-4222. doi: 10.1007/s00018-020-03519-6. Epub 2020 Apr 8.
2
Genetic Analysis of Protein Translocation.蛋白质易位的遗传分析。
Protein J. 2019 Jun;38(3):217-228. doi: 10.1007/s10930-019-09813-y.
3
Evidence for the transport of maltose by the sucrose permease, CscB, of Escherichia coli.大肠杆菌蔗糖通透酶CscB转运麦芽糖的证据。
J Membr Biol. 2009 Mar;228(2):79-88. doi: 10.1007/s00232-009-9161-9. Epub 2009 Mar 18.
4
Amino acids that confer transport of raffinose and maltose sugars in the raffinose permease (RafB) of Escherichia coli as implicated by spontaneous mutations at Val-35, Ser-138, Ser-139, Gly-389 and Ile-391.缬氨酸-35、丝氨酸-138、丝氨酸-139、甘氨酸-389和异亮氨酸-391处的自发突变表明，在大肠杆菌棉子糖通透酶（RafB）中赋予棉子糖和麦芽糖转运功能的氨基酸。
J Membr Biol. 2007 Dec;220(1-3):87-95. doi: 10.1007/s00232-007-9077-1. Epub 2007 Nov 17.
5
Altered substrate selection of the melibiose transporter (MelY) of Enterobacter cloacae involving point mutations in Leu-88, Leu-91, and Ala-182 that confer enhanced maltose transport.阴沟肠杆菌蜜二糖转运蛋白（MelY）的底物选择改变，涉及亮氨酸-88、亮氨酸-91和丙氨酸-182的点突变，这些突变导致麦芽糖转运增强。
J Bacteriol. 2003 Jun;185(12):3672-7. doi: 10.1128/JB.185.12.3672-3677.2003.
6
Unliganded maltose-binding protein triggers lactose transport in an Escherichia coli mutant with an alteration in the maltose transport system.未结合配体的麦芽糖结合蛋白在麦芽糖转运系统发生改变的大肠杆菌突变体中触发乳糖转运。
J Bacteriol. 1997 Dec;179(24):7687-94. doi: 10.1128/jb.179.24.7687-7694.1997.
7
Lactose carrier mutants of Escherichia coli with changes in sugar recognition (lactose versus melibiose).大肠杆菌乳糖载体突变体，其糖识别发生改变（乳糖与蜜二糖）。
J Bacteriol. 1997 Sep;179(17):5570-3. doi: 10.1128/jb.179.17.5570-5573.1997.
8
Lactose permease mutants which transport (malto)-oligosaccharides.转运（麦芽）寡糖的乳糖通透酶突变体。
J Bacteriol. 1993 Oct;175(19):6269-75. doi: 10.1128/jb.175.19.6269-6275.1993.
9
Formation and excretion of acetylmaltose after accumulation of maltose in Escherichia coli.麦芽糖在大肠杆菌中积累后乙酰麦芽糖的形成与排泄。
J Bacteriol. 1981 May;146(2):725-32. doi: 10.1128/jb.146.2.725-732.1981.
10
Mutations in the lacY gene of Escherichia coli define functional organization of lactose permease.大肠杆菌乳糖通透酶基因lacY的突变确定了乳糖通透酶的功能组织。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7652-6. doi: 10.1073/pnas.78.12.7652.
本文引用的文献
1
[Galactoside-permease of Escherichia coli].[大肠杆菌的半乳糖苷通透酶]
Ann Inst Pasteur (Paris). 1956 Dec;91(6):829-57.
2
Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli.大肠杆菌β-半乳糖苷转运系统组分M蛋白的特异性标记与部分纯化
Proc Natl Acad Sci U S A. 1965 Sep;54(3):891-9. doi: 10.1073/pnas.54.3.891.
3
[Isolation of membrane mutants in Escherichia coli].[大肠杆菌膜突变体的分离]
C R Acad Hebd Seances Acad Sci D. 1970 May 25;270(21):2591-3.
4
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.阿拉伯糖操纵子的定向转座：一种用于分离针对任何大肠杆菌基因的特异性转导噬菌体的技术。
J Mol Biol. 1969 Aug 28;44(1):117-27. doi: 10.1016/0022-2836(69)90408-2.
5
A second transport system for L-arabinose in Escherichia coli B-r controlled by the araC gene.大肠杆菌B-r中由araC基因控制的L-阿拉伯糖的第二种转运系统。
J Bacteriol. 1972 Aug;111(2):606-13. doi: 10.1128/jb.111.2.606-613.1972.
6
Lactose permeation via the arabinose transport system in Escherichia coli K-12.通过大肠杆菌K-12中的阿拉伯糖转运系统进行乳糖渗透。
J Bacteriol. 1974 Oct;120(1):266-72. doi: 10.1128/jb.120.1.266-272.1974.
7
Divergent operons and the genetic structure of the maltose B region in Escherichia coli K12.大肠杆菌K12中不同的操纵子与麦芽糖B区域的遗传结构
Genetics. 1974 Feb;76(2):169-84. doi: 10.1093/genetics/76.2.169.
8
Dierect measurement of the binding of labeled sugars to the lactose permease M protein.直接测量标记糖与乳糖通透酶M蛋白的结合。
不过你原文中“Dierect”拼写有误，应该是“Direct”。
J Biol Chem. 1974 Jan 10;249(1):33-7.
9
malB region in Escherichia coli K-12: characterization of new mutations.大肠杆菌K-12中的malB区域：新突变的特征分析
J Bacteriol. 1974 Jan;117(1):40-7. doi: 10.1128/jb.117.1.40-47.1974.
10
Active transport of maltose in Escherichia coli K12. Involvement of a "periplasmic" maltose binding protein.大肠杆菌K12中麦芽糖的主动运输。一种“周质”麦芽糖结合蛋白的作用。
Eur J Biochem. 1974 Aug 15;47(1):139-49. doi: 10.1111/j.1432-1033.1974.tb03677.x.
Zotero 插件