Mouches C, Candresse T, Gadeau A, Barroso G, Saillard C, Wroblewski H, Bove J M
Isr J Med Sci. 1984 Sep;20(9):773-7.
Expression in Escherichia coli of the cloned Spiroplasma citri spiralin gene results in the expression of a 30.5-kDa protein serologically related to spiralin. A protein with the same properties is also present in minor amounts in S. citri cells, suggesting that spiralin is first produced as a preprotein containing a signal polypeptide, which is removed during further processing. Hybridization experiments have demonstrated that pES1, the recombinant plasmid carrying the spiralin gene, can be used as a molecular probe, allowing the detection of S. citri DNA in infected plants and in insect cell cultures.
克隆的柑橘螺原体螺旋蛋白基因在大肠杆菌中的表达导致了一种与螺旋蛋白血清学相关的30.5 kDa蛋白的表达。具有相同特性的一种蛋白也少量存在于柑橘螺原体细胞中，这表明螺旋蛋白最初是以包含信号多肽的前体蛋白形式产生的，该信号多肽在进一步加工过程中被去除。杂交实验表明，携带螺旋蛋白基因的重组质粒pES1可用作分子探针，用于检测受感染植物和昆虫细胞培养物中的柑橘螺原体DNA。
