Saifuku K, Sekine T, Namihisa T, Takahashi T, Kanaoka Y
Clin Chim Acta. 1978 Mar 1;84(1-2):85-91. doi: 10.1016/0009-8981(78)90479-5.
A simple and rapid rate assay of serum leucine aminopeptidase is described, using a novel fluorogenic substrate, 7-L-leucyl-4-methylcoumarinylamide. The reaction is initiated by adding 10 microliter of serum, and the fluorescence development for 1 min due to the 7-amino-4-methylcoumarin liberated at 37 degrees C is followed directly on a recorder. The proposed method is proved to be free from error due to the adsorption of the substrate dye to serum albumin and to be applicable to hyperbilirubinemic sera by simple correction. The values obtained by this method showed good correlation with those obtained by the conventional method of Goldbarg and Rutenberg (Goldbarg, J.A. and Rutenberg, A.M. (1958) Cancer 11, 283-291).
本文描述了一种简单快速的血清亮氨酸氨基肽酶速率测定法，该方法使用了一种新型荧光底物7-L-亮氨酰-4-甲基香豆素酰胺。通过加入10微升血清启动反应，并在记录仪上直接跟踪37℃下由于释放出7-氨基-4-甲基香豆素而产生的1分钟荧光发展情况。结果表明，所提出的方法不存在底物染料吸附到血清白蛋白上导致的误差，并且通过简单校正适用于高胆红素血症血清。用该方法获得的值与通过Goldbarg和Rutenberg的传统方法（Goldbarg, J.A.和Rutenberg, A.M. (1958) Cancer 11, 283 - 291）获得的值具有良好的相关性。
