Suzuki M, Ueno T, Takahashi T, Kanaoka Y, Okuyama T, Furuya H, Sekine T
Clin Chim Acta. 1981 Sep 10;115(2):223-8. doi: 10.1016/0009-8981(81)90078-4.
A rapid and sensitive fluorometric rate assay of serum cystine aminopeptidase is described using a novel fluorogenic substrate, 7-S-benzyl-L-cysteinyl-4-methylcoumarinylamide. The proposed method enabled us to determine cystine aminopeptidase activity in one minute using 10 microliter of serum. The assay was shown to be free from error due to the adsorption of the substrate to serum albumin. The values obtained by this method gave good correlation with those obtained by the conventional method of Watson [6].
本文描述了一种使用新型荧光底物7-S-苄基-L-半胱氨酰-4-甲基香豆素酰胺对血清胱氨酸氨肽酶进行快速灵敏的荧光速率测定法。所提出的方法使我们能够用10微升血清在一分钟内测定胱氨酸氨肽酶活性。该测定法显示不受底物吸附到血清白蛋白的影响。通过该方法获得的值与通过沃森[6]的传统方法获得的值具有良好的相关性。
