与光系统II相关多肽的放射性同位素证据。

Radioisotopic evidence for the polypeptides associated with photosystem II.

作者信息

Daniell H, Krishnan M, Ranganathan M, Gnanam A

出版信息

Biochem Biophys Res Commun. 1984 Dec 28;125(3):988-95. doi: 10.1016/0006-291x(84)91381-0.

Abstract

Direct radioisotopic evidence for the polypeptides associated with oxygen evolution in a non-destructive approach by comparing the polypeptides of photosystem II (PS II) particles in etioplasts that lack O2 evolution with that of chloroplasts that exhibit high rates of oxygen evolution is reported. Polypeptide analysis by coomassie blue staining revealed that 32, 23 and 15 kDa proteins were absent in etioplast particles but developed in chloroplast PS II particles after illumination. However, a strikingly different picture was obtained when labelled polypeptides were analyzed from the fluorograph. While the new proteins identified by coomassie blue staining were also labelled, intensely labelled major polypeptides of molecular range 40, 41, 43 kD and minor polypeptides of molecular range 47, 48, 51 kD were observed in chloroplast PS II particles but not in etioplasts or etioplast particles; these labelled polypeptides were so prominent that they could be identified even in thylakoid membranes.

摘要

报道了通过比较缺乏氧气释放能力的黄化质体中光系统II（PS II）颗粒的多肽与具有高氧气释放速率的叶绿体中PS II颗粒的多肽，以无损方法获得与氧气释放相关的多肽的直接放射性同位素证据。考马斯亮蓝染色的多肽分析表明，32 kDa、23 kDa和15 kDa的蛋白质在黄化质体颗粒中不存在，但在光照后在叶绿体PS II颗粒中出现。然而，当从荧光自显影片分析标记的多肽时，得到了截然不同的结果。虽然考马斯亮蓝染色鉴定出的新蛋白质也被标记，但在叶绿体PS II颗粒中观察到分子范围为40、41、43 kD的强标记主要多肽以及分子范围为47、48、51 kD的弱标记多肽，而在黄化质体或黄化质体颗粒中未观察到；这些标记的多肽非常突出，甚至在类囊体膜中也能识别。