White M J, Green B R
Eur J Biochem. 1987 Jun 15;165(3):531-5. doi: 10.1111/j.1432-1033.1987.tb11471.x.
Polypeptides of the three major chlorophyll a + b protein complexes were detected in a chlorophyll-b-less barley mutant (chlorina f2) using immunological techniques. Antibodies to CP Ia, a photosystem I complex containing both the reaction center (CP I) and the chlorophyll a + b antenna (LHCI), detected substantial amounts of LHCI polypeptides in mutant thylakoids. Some polypeptides of the two photosystem-II-associated chlorophyll a + b complexes, CP 29 and LHCII, were also detected using antibodies raised against these complexes. The CP 29 apoprotein and the minor 25-kDa polypeptide of LHCII were present in amounts that could be seen by Coomassie blue staining. In contrast, the two major polypeptides of LHCII were greatly diminished in amount, and one of them may be completely absent. These data suggest that the absence of chlorophyll b may have differing effects on the synthesis, processing or turnover of the various chlorophyll a + b binding polypeptides. They also show that these polypeptides can be inserted into thylakoids in the absence of Chl b, and that significant amounts of some of them are accumulated in the mutant thylakoids.
利用免疫学技术，在一种缺乏叶绿素b的大麦突变体（叶绿素缺乏f2）中检测到了三种主要叶绿素a + b蛋白复合物的多肽。针对CP Ia的抗体，CP Ia是一种包含反应中心（CP I）和叶绿素a + b天线（LHCI）的光系统I复合物，在突变体类囊体中检测到了大量的LHCI多肽。还使用针对这两种与光系统II相关的叶绿素a + b复合物CP 29和LHCII产生的抗体检测到了它们的一些多肽。CP 29脱辅基蛋白和LHCII的25 kDa小多肽的含量通过考马斯亮蓝染色可见。相比之下，LHCII的两种主要多肽的含量大大减少，其中一种可能完全不存在。这些数据表明，叶绿素b的缺失可能对各种叶绿素a + b结合多肽的合成、加工或周转有不同的影响。它们还表明，在没有叶绿素b的情况下，这些多肽可以插入类囊体中，并且其中一些在突变体类囊体中大量积累。
