通过向HAT培养基（HIAT）中添加胰岛素来增强杂交瘤形成。

Enhancement of hybridoma formation by addition of insulin to HAT medium (HIAT).

作者信息

Feit C, Bartal A H, Hirshaut Y

出版信息

Hybridoma. 1984 Winter;3(4):377-80. doi: 10.1089/hyb.1984.3.377.

DOI:10.1089/hyb.1984.3.377

Abstract

The effects of insulin on the formation of hybridoma clones following fusion experiments with SRBC immunized BALB/c mouse spleen cells and P3U1 mouse plasmacytoma cells were evaluated. The addition of insulin to HAT medium (HIAT) resulted in significant increases in the number and size of hybridoma colonies generated. The total number of anti-SRBC antibody-secreting clones also increased as much as sevenfold using insulin-supplemented medium compared to HAT alone. In view of the increasing interest in hybridoma technology for monoclonal antibody production, the use of insulin-supplemented medium (HIAT) may significantly expedite ongoing work by providing a more efficient method for the establishment of stable clones.

摘要

评估了胰岛素对用经绵羊红细胞（SRBC）免疫的BALB/c小鼠脾细胞和P3U1小鼠浆细胞瘤细胞进行融合实验后杂交瘤克隆形成的影响。向次黄嘌呤氨基蝶呤胸腺嘧啶核苷培养基（HAT）中添加胰岛素（HIAT）后，产生的杂交瘤集落数量和大小显著增加。与单独使用HAT相比，使用添加胰岛素的培养基时，分泌抗SRBC抗体的克隆总数增加了多达7倍。鉴于对用于单克隆抗体制备的杂交瘤技术的兴趣日益浓厚，使用添加胰岛素的培养基（HIAT）可能通过提供一种更有效的建立稳定克隆的方法，显著加快正在进行的工作。