针对大环单端孢霉烯族毒素罗里菌素A的单克隆抗体的制备与特性分析
Production and characterization of monoclonal antibodies to the macrocyclic trichothecene roridin A.
作者信息
Hack R, Märtlbauer E, Terplan G
机构信息
Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, Federal Republic of Germany.
出版信息
Appl Environ Microbiol. 1988 Sep;54(9):2328-30. doi: 10.1128/aem.54.9.2328-2330.1988.
Two murine monoclonal antibodies to the macrocyclic trichothecene roridin A are described. Screening for antibody production was performed on absorbed anti-mouse immunoglobulin serum as double-antibody solid phase, and further characterization was done on affinity-purified anti-mouse IgG serum. The antibodies, designated 5G11 and 4H10, had affinity constants for roridin A of 9.25 X 10(7) and 1.7 X 10(7) liters/mol, respectively. In monoclonal antibody-based direct enzyme immunoassays, these IgG1 antibodies had detection limits for roridin A of 0.4 ng/ml (0.02 ng per assay) and 1.8 ng/ml (0.09 ng per assay), respectively. Both antibodies were most specific for the tested macrocyclic trichothecenes. The relative cross-reactivities of antibody 5G11 with roridin A, roridin J, verrucarin A, satratoxin G, and satratoxin H were 100.0, 43.8, 16.7, 3.7, and 18.9%, respectively; for antibody 4H10 they were 100.0, 6.3, 64.0, 4.4, and 4.9%, respectively.
描述了两种针对大环单端孢霉烯族毒素洛里菌素A的鼠单克隆抗体。以吸收的抗小鼠免疫球蛋白血清作为双抗体固相进行抗体产生的筛选，并对亲和纯化的抗小鼠IgG血清进行进一步表征。命名为5G11和4H10的抗体对洛里菌素A的亲和常数分别为9.25×10⁷和1.7×10⁷升/摩尔。在基于单克隆抗体的直接酶免疫测定中，这些IgG1抗体对洛里菌素A的检测限分别为0.4 ng/ml（每次测定0.02 ng）和1.8 ng/ml（每次测定0.09 ng）。两种抗体对所测试的大环单端孢霉烯族毒素具有最高特异性。抗体5G11与洛里菌素A、洛里菌素J、疣孢菌素A、葡萄穗霉毒素G和葡萄穗霉毒素H的相对交叉反应率分别为100.0%、43.8%、16.7%、3.7%和18.9%；抗体4H10的相对交叉反应率分别为100.0%、6.3%、64.0%、4.4%和4.9%。
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