Korn-Lubetzki I, Ovadia H, Wald U, Abramsky O
Neurosci Lett. 1986 Aug 15;69(1):25-30. doi: 10.1016/0304-3940(86)90408-8.
Based on the successful use of a basement membrane-like extracellular matrix (ECM) as a physiological substrate for the growth of adult rat oligodendrocytes, we have applied the system to oligodendroglia of human origin. ECM is excreted and deposited on a culture dish by endothelia cells. Glial cells isolated from fresh human brains by a Percoll density gradient were maintained on ECM for several weeks in vitro. Cells identified as oligodendrocytes by anti-galactocerebroside (GalC) could be transferred and replated on ECM-coated plates. In one case, GalC-positive cells isolated from a biopsy of an 8-year old girl were able to incorporate [3H]thymidine as revealed by autoradiography. ECM can be used successfully for the growth and in vitro maintenance of human oligodendrocytes.
基于成功地将一种基底膜样细胞外基质（ECM）用作成年大鼠少突胶质细胞生长的生理底物，我们已将该系统应用于人类来源的少突胶质细胞。ECM由内皮细胞分泌并沉积在培养皿上。通过Percoll密度梯度从新鲜人脑分离的神经胶质细胞在体外于ECM上维持数周。通过抗半乳糖脑苷脂（GalC）鉴定为少突胶质细胞的细胞能够转移并重新接种到涂有ECM的培养板上。在一个案例中，从一名8岁女孩的活检组织中分离出的GalC阳性细胞能够掺入[3H]胸腺嘧啶核苷，放射自显影显示了这一点。ECM可成功用于人类少突胶质细胞的生长和体外维持。
