Williams R N, Paterson C A
Eur J Pharmacol. 1984 Oct 30;106(1):113-9. doi: 10.1016/0014-2999(84)90684-8.
The effect of anti-inflammatory corticosteroids upon leukocyte infiltration was investigated in vivo, using the eye as an experimental model. The inflammatory response was induced by injecting bacterial endotoxin into the vitreous body of the albino rabbit eye. Polymorphonuclear leukocyte (PMN) infiltration into the aqueous humor was quantified by counting cells directly, whereas accumulation of leukocytes in the ocular tissues was determined by measuring myeloperoxidase (MPO) activity. Systemically administered corticosteroids inhibited plasma protein leakage and PMN infiltration into the aqueous humor, but, anomalously, increased MPO activity and hence the number of PMNs in the ciliary body. These findings were confirmed qualitatively by histology. However, the clinical manifestations of the inflammatory responses were almost totally suppressed by the corticosteroids. We suggest that the inhibitory effect of corticosteroids on PMN infiltration into the aqueous humor, and possibly inflammatory exudates in general, represents the suppression of inflammatory cell activity in a manner which is unrelated to a direct effect on leukocyte movement.
以眼睛作为实验模型，在体内研究了抗炎皮质类固醇对白细胞浸润的影响。通过向白化兔眼玻璃体内注射细菌内毒素诱导炎症反应。通过直接计数细胞来量化多形核白细胞（PMN）向房水的浸润，而通过测量髓过氧化物酶（MPO）活性来确定白细胞在眼组织中的积聚。全身给予皮质类固醇可抑制血浆蛋白渗漏和PMN向房水的浸润，但反常的是，会增加MPO活性，从而增加睫状体中PMN的数量。组织学定性证实了这些发现。然而，皮质类固醇几乎完全抑制了炎症反应的临床表现。我们认为，皮质类固醇对PMN向房水浸润以及可能对一般炎症渗出物的抑制作用，是以一种与对白细胞运动的直接作用无关的方式抑制炎症细胞活性。
