Eva C, Hadjiconstantinou M, Neff N H, Meek J L
Anal Biochem. 1984 Dec;143(2):320-4. doi: 10.1016/0003-2697(84)90670-5.
Choline oxidase and cholinesterase were found to retain their activity for 1-2 weeks at room temperature while adsorbed to a commercially available anion-exchange cartridge. These enzymes convert acetylcholine to H2O2. Acetylcholine can be measured in tissue extracts by separation at pH 7 on a polymeric reverse-phase high-performance liquid chromatography column, conversion of acetylcholine to H2O2 on a postcolumn enzyme-loaded anion-exchange cartridge, and electrochemical detection of the H2O2 formed.
胆碱氧化酶和胆碱酯酶吸附到市售阴离子交换柱上时，在室温下可保持其活性1至2周。这些酶将乙酰胆碱转化为过氧化氢。乙酰胆碱可通过在聚合物反相高效液相色谱柱上于pH 7进行分离、在柱后装有酶的阴离子交换柱上把乙酰胆碱转化为过氧化氢以及对生成的过氧化氢进行电化学检测，从而在组织提取物中进行测定。
