Ohneda A, Kobayashi T, Nihei J
Horm Metab Res. 1984 Dec;16 Suppl 1:105-9. doi: 10.1055/s-2007-1014910.
Previous study from our laboratory demonstrated that an increased release of gut glucagon-like immunoreactivity (GLI) following triglyceride digestion was not observed in pancreatectomized dogs. Therefore, in order to clarify the response of gut GLI to triglyceride ingestion, experimental study was carried out in normal and pancreatectomized dogs. When butter was administered to pancreatectomized dogs in combination with pancreatic enzymes, plasma glucagon (IRG) measured by specific antiserum did not change significantly but plasma triglyceride as well as plasma total immunoreactive glucagon (total IRG) measured by nonspecific antiserum increased. Oral administration of glycerol induced no significant increase in plasma IRG but elicited a moderate increase of plasma total IRG. Gastric instillation of palmitic acid induced a remarkable rise of plasma total IRG in normal dogs, whereas plasma IRG did not reveal any changes. Oral administration of triglyceride of medium chain fatty acid, tricaprylin, did not promote any rise of plasma IRG but a slight increase of plasma total IRG. From the present experiment, it is concluded that hydrolysis of triglyceride and its metabolites are important in the release of gut GLI following fat ingestion. Among its metabolites, long chain fatty acid and glycerol promote the GLI secretion, whereas medium chain fatty acid does not.
我们实验室之前的研究表明，在胰腺切除的犬类中未观察到甘油三酯消化后肠道胰高血糖素样免疫反应性（GLI）释放增加。因此，为了阐明肠道GLI对甘油三酯摄入的反应，我们对正常犬和胰腺切除犬进行了实验研究。当给胰腺切除的犬类喂食黄油并同时给予胰酶时，用特异性抗血清测量的血浆胰高血糖素（IRG）没有显著变化，但用非特异性抗血清测量的血浆甘油三酯以及血浆总免疫反应性胰高血糖素（总IRG）增加。口服甘油不会使血浆IRG显著增加，但会使血浆总IRG适度增加。向正常犬胃内滴注棕榈酸会导致血浆总IRG显著升高，而血浆IRG没有任何变化。口服中链脂肪酸甘油三酯三辛酸甘油酯不会使血浆IRG升高，但会使血浆总IRG略有增加。从目前的实验可以得出结论，甘油三酯及其代谢产物的水解在脂肪摄入后肠道GLI的释放中很重要。在其代谢产物中，长链脂肪酸和甘油会促进GLI分泌，而中链脂肪酸则不会。
