Ohneda A, Sasaki I, Naito H, Toda M, Ohneda M, Koizumi F
First Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Am J Physiol. 1989 Mar;256(3 Pt 1):E431-8. doi: 10.1152/ajpendo.1989.256.3.E431.
Previous studies demonstrated that insulin-induced hypoglycemia enhances glicentin secretion in piglets and prompted us to investigate the response of glucagon-like immunoreactivity (GLI) to hypoglycemia in dogs. Insulin hypoglycemia did not induce any rise of plasma total immunoreactive glucagon (IRG) measured by nonspecific antiserum to glucagon in normal or pancreatectomized dogs under anesthesia. In contrast, insulin-induced hypoglycemia clearly increased plasma total IRG in both normal and pancreatectomized dogs in a conscious state. Administration of acetylcholine resulted in an elevation of plasma total IRG, whereas epinephrine induced a slight increase in plasma total IRG. The infusion of alpha- or beta-adrenergic blockers did not affect the response of plasma total IRG to hypoglycemia, whereas atropine completely blunted the increase in plasma total IRG during insulin hypoglycemia. Similarly atropine abolished the rise of plasma total IRG during intravenous administration of 2-deoxy-D-glucose. It is concluded that hypoglycemia clearly enhances the secretion of GLI from the gut in dogs and that GLI secretion during hypoglycemia is modulated, at least in part, by the autonomic nervous system.
先前的研究表明，胰岛素诱导的低血糖会增强仔猪的胰高血糖素样肽分泌，这促使我们研究犬类中胰高血糖素样免疫反应性（GLI）对低血糖的反应。在麻醉状态下，胰岛素低血糖并未使正常犬或胰腺切除犬中通过对胰高血糖素的非特异性抗血清测量的血浆总免疫反应性胰高血糖素（IRG）升高。相反，在清醒状态下，胰岛素诱导的低血糖明显增加了正常犬和胰腺切除犬的血浆总IRG。给予乙酰胆碱导致血浆总IRG升高，而肾上腺素引起血浆总IRG略有增加。输注α-或β-肾上腺素能阻滞剂不影响血浆总IRG对低血糖的反应，而阿托品完全消除了胰岛素低血糖期间血浆总IRG的增加。同样，阿托品消除了静脉注射2-脱氧-D-葡萄糖期间血浆总IRG的升高。结论是，低血糖明显增强了犬类肠道中GLI的分泌，并且低血糖期间GLI的分泌至少部分受自主神经系统调节。
