Rutherford A, Burnstock G
Eur J Pharmacol. 1978 Mar 15;48(2):195-202. doi: 10.1016/0014-2999(78)90328-x.
Guinea-pig taenia coli preprations were pre-incubated in 3H-adenosine and the overflow of 3H-compounds to a superfusing medium was measured during transmural field stimulation in the presence and absence of tetrodotoxin. The fractional overflow of 3H-compounds on stimulation of taenia coli pre-incubated in 3H-adenosine at 10(-7) M was greater than after incubation at 10(-5) M. About half of the releasable store was blocked by tetrodotoxin. It is proposed that at lower concentrations, there is selective loading of a releasable store, while at higher concentrations thare is a greater loading of non-releasable stores, and that neuronal stores account for about 33% of the 3H-compounds released by transmural stimulation.
将豚鼠结肠带制备物在3H-腺苷中预孵育，在有无河豚毒素的情况下，通过跨壁电场刺激来测量3H化合物向灌注培养基中的溢出量。在10^(-7)M的3H-腺苷中预孵育的结肠带受到刺激时，3H化合物的分数溢出量大于在10^(-5)M孵育后的情况。约一半的可释放储备被河豚毒素阻断。有人提出，在较低浓度下，有选择性地加载可释放储备，而在较高浓度下，不可释放储备的加载量更大，并且神经元储备约占跨壁刺激释放的3H化合物的33%。
