Portier M M, de Néchaud B, Gros F
Dev Neurosci. 1983;6(6):335-44. doi: 10.1159/000112360.
Peripherin, a Triton-insoluble protein, whose distribution was found to be restricted to neurons in the rodent and human peripheral nervous system, was characterized by its electrophoretic features (isoelectric point: 5.6; molecular weight: 56,000 daltons) and by its peptidic map after limited proteolysis. Comparative peptide analysis of the 70,000-dalton subunit of neurofilaments (70K NFP), vimentin and peripherin, was performed by two different methods; limited proteolysis with Staphylococcus aureus V8 protease yields a different peptidic map for each protein; treatment with N-chlorosuccinimide, which cleaves preferentially at tryptophan residues, yields only two peptides from each protein: the size of the two fragments indicates that these proteins possess a single tryptophan residue located in the central part of the molecule. A rabbit antiserum raised against mouse peripherin decorated an intracellular filamentous network in mouse neuroblastoma NIE 115 cell line. The IgG fraction of the antiserum recognizes peripherin and the smallest subunit of the neurofilament triplet (70K NFP)--but not vimentin--whereas a monoclonal anti-70K NFP recognizes only the 70K NFP. Moreover, peripherin displays the common antigenic determinant shared by all intermediate filament proteins. Hence, we propose that peripherin represents a new member of the intermediate filament protein family, and might belong to the neurofilament class.
外周蛋白是一种不溶于曲通的蛋白质，其分布仅限于啮齿动物和人类外周神经系统的神经元，通过其电泳特征（等电点：5.6；分子量：56,000道尔顿）和有限蛋白酶解后的肽图来表征。通过两种不同方法对神经丝70,000道尔顿亚基（70K NFP）、波形蛋白和外周蛋白进行了比较肽分析；用金黄色葡萄球菌V8蛋白酶进行有限蛋白酶解，每种蛋白质产生不同的肽图；用N - 氯代琥珀酰亚胺处理，其优先在色氨酸残基处切割，每种蛋白质仅产生两个肽段：两个片段的大小表明这些蛋白质在分子中部有一个单一的色氨酸残基。用针对小鼠外周蛋白产生的兔抗血清标记了小鼠神经母细胞瘤NIE 115细胞系中的细胞内丝状网络。抗血清的IgG部分识别外周蛋白和神经丝三联体的最小亚基（70K NFP）——但不识别波形蛋白——而单克隆抗70K NFP仅识别70K NFP。此外，外周蛋白显示出所有中间丝蛋白共有的共同抗原决定簇。因此，我们提出外周蛋白代表中间丝蛋白家族的一个新成员，可能属于神经丝类别。
